The Three Rs of Single-Cell RNA Sequencing: Reuse, Refine, and Resource.
Quan M Phan, Iwona M Driskell, Ryan R Driskell
Author Information
Quan M Phan: School of Molecular Biosciences, Washington State University, Pullman, Washington, USA.
Iwona M Driskell: School of Molecular Biosciences, Washington State University, Pullman, Washington, USA.
Ryan R Driskell: School of Molecular Biosciences, Washington State University, Pullman, Washington, USA; Center for Reproductive Biology, Washington State University, Pullman, Washington, USA. Electronic address: ryan.driskell@wsu.edu.
Single-cell RNA sequencing (scRNA-seq) provides an unprecedented ability to investigate cellular heterogeneity in entire organs and tissues, including human skin. Ascensión et al. (2020) combined and reanalyzed human skin scRNA-seq datasets to uncover new insights into fibroblast heterogeneity. This work demonstrates that new discoveries can be made from published data on the basis of principles of these three Rs: Reuse, Refine, and Resource.
References
Dev Cell. 2015 Sep 14;34(5):577-91
[PMID: 26256211]
