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International journal of molecular sciences
Jun 17, 2021;22 (12):

Differential Regulation of Gonadotropins as Revealed by Transcriptomes of Distinct LH and FSH Cells of Fish Pituitary.

Lian Hollander-Cohen, Matan Golan, Berta Levavi-Sivan
Author Information
  1. Lian Hollander-Cohen: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel.
  2. Matan Golan: Department of Poultry and Aquaculture, Institute of Animal Sciences, Agricultural Research Organization, Volcani Center, P.O.B 15159, Rishon Letziyon 7505101, Israel. ORCID
  3. Berta Levavi-Sivan: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel. ORCID
PMID: 34204216 DOI: 10.3390/ijms22126478

Abstract

From mammals to fish, reproduction is driven by luteinizing hormone (LH) and follicle-stimulating hormone (FSH) temporally secreted from the pituitary gland. Teleost fish are an excellent model for addressing the unique regulation and function of each gonadotropin cell since, unlike mammals, they synthesize and secrete LH and FSH from distinct cells. Only very distant vertebrate classes (such as fish and birds) demonstrate the mono-hormonal strategy, suggesting a potential convergent evolution. Cell-specific transcriptome analysis of double-labeled transgenic tilapia expressing GFP and RFP in LH or FSH cells, respectively, yielded genes specifically enriched in each cell type, revealing differences in hormone regulation, receptor expression, cell signaling, and electrical properties. Each cell type expresses a unique GPCR signature that reveals the direct regulation of metabolic and homeostatic hormones. Comparing these novel transcriptomes to that of rat gonadotrophs revealed conserved genes that might specifically contribute to each gonadotropin activity in mammals, suggesting conserved mechanisms controlling the differential regulation of gonadotropins in vertebrates.

Keywords

FSH GPCR GnRH LH cck gonadotropins neuropeptides pituitary

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Grants

  1. PF683/5-1/Deutsche Forschungsgemeinschaft
  2. 1540/17/Israel Science Foundation

MeSH Term

Animals
Biomarkers
Cell Separation
Computational Biology
Fishes
Fluorescent Antibody Technique
Follicle Stimulating Hormone
Gene Expression Profiling
Gene Expression Regulation
Gonadotropins
Luteinizing Hormone
Molecular Sequence Annotation
Phylogeny
Pituitary Gland
Rats

Chemicals

Biomarkers
Gonadotropins
Luteinizing Hormone
Follicle Stimulating Hormone
Journal Article
Article has an altmetric score of 5

Word Cloud

Created with Highcharts 10.0.0LHFSHregulationcellmammalsfishhormonepituitaryuniquegonadotropincellssuggestinggenesspecificallytypeGPCRconservedgonadotropinsreproductiondrivenluteinizingfollicle-stimulatingtemporallysecretedglandTeleostexcellentmodeladdressingfunctionsinceunlikesynthesizesecretedistinctdistantvertebrateclassesbirdsdemonstratemono-hormonalstrategypotentialconvergentevolutionCell-specifictranscriptomeanalysisdouble-labeledtransgenictilapiaexpressingGFPRFPrespectivelyyieldedenrichedrevealingdifferencesreceptorexpressionsignalingelectricalpropertiesexpressessignaturerevealsdirectmetabolichomeostatichormonesComparingnoveltranscriptomesratgonadotrophsrevealedmightcontributeactivitymechanismscontrollingdifferentialvertebratesDifferentialRegulationGonadotropinsRevealedTranscriptomesDistinctCellsFishPituitaryGnRHcckneuropeptides

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