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ACS chemical biology
08 20, 2021;16 (8): 1344-1349.

Epitope Recognition of a Monoclonal Antibody Raised against a Synthetic Glycerol Phosphate Based Teichoic Acid.

Francesca Berni, Ermioni Kalfopoulou, Ana M Gimeno Cardells, Filippo Carboni, Daan van der Es, Felipe Romero-Saavedra, Diana Laverde, Karmela Miklic, Suzana Malic, Tihana L Rovis, Stipan Jonjic, Sara Ali, Herman S Overkleeft, Cornelis H Hokke, Angela van Diepen, Roberto Adamo, Jesús Jiménez-Barbero, Gijsbert A van der Marel, Johannes Huebner, Jeroen D C Codée
Author Information
  1. Francesca Berni: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. ORCID
  2. Ermioni Kalfopoulou: Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, 80337 Munich, Germany.
  3. Ana M Gimeno Cardells: CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, 48160 Derio, Spain.
  4. Filippo Carboni: Research and Development Centre, GlaxoSmithKline (GSK), 53100 Siena, Italy.
  5. Daan van der Es: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. ORCID
  6. Felipe Romero-Saavedra: Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, 80337 Munich, Germany.
  7. Diana Laverde: Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, 80337 Munich, Germany.
  8. Karmela Miklic: Centre for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  9. Suzana Malic: Centre for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  10. Tihana L Rovis: Centre for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  11. Stipan Jonjic: Centre for Proteomics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia.
  12. Sara Ali: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
  13. Herman S Overkleeft: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
  14. Cornelis H Hokke: Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands. ORCID
  15. Angela van Diepen: Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
  16. Roberto Adamo: Research and Development Centre, GlaxoSmithKline (GSK), 53100 Siena, Italy. ORCID
  17. Jesús Jiménez-Barbero: CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, 48160 Derio, Spain. ORCID
  18. Gijsbert A van der Marel: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
  19. Johannes Huebner: Division of Pediatric Infectious Diseases, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University, 80337 Munich, Germany.
  20. Jeroen D C Codée: Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. ORCID
PMID: 34255482 DOI: 10.1021/acschembio.1c00422

Abstract

Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR-analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.

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MeSH Term

Animals
Antibodies, Monoclonal, Murine-Derived
Enzyme-Linked Immunosorbent Assay
Epitopes
Glycerophosphates
Mice
Nuclear Magnetic Resonance, Biomolecular
Protein Binding
Teichoic Acids

Chemicals

Antibodies, Monoclonal, Murine-Derived
Epitopes
Glycerophosphates
Teichoic Acids
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

Created with Highcharts 10.0.0GlycerolGroPusingsyntheticinteractionantibodyTAbindingphosphate-basedteichoicacidsTAsantigeniccell-wallcomponentsfoundenterococcusstaphylococcusspeciesimmunogenicityexplorednativestructuresdetailsyetreportedstructuralbasisantibodiesworkrepresentsfirstcasestudymonoclonalgenerateddevelopedemployedmolecular-levelanalysismicroarraysELISASPR-analysesSTD-NMRspectroscopyfindingsshownumberchiralityresiduescrucialsugarappendagecontributespresentationbackbonesiteEpitopeRecognitionMonoclonalAntibodyRaisedSyntheticPhosphateBasedTeichoicAcid

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