Changes of DNA Damage Effect of T-2 or Deoxynivalenol Toxins during Three Weeks Exposure in Common Carp ( L.) Revealed by LORD-Q PCR.
Rubina Tünde Szabó, Mária Kovács-Weber, Krisztián Milán Balogh, Miklós Mézes, Balázs Kovács
Author Information
Rubina Tünde Szabó: Institute of Animal Husbandry, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary.
Mária Kovács-Weber: Institute of Animal Husbandry, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary.
Krisztián Milán Balogh: Department of Feed Toxicology, Institute of Physiology and Nutrition, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary. ORCID
Miklós Mézes: Department of Feed Toxicology, Institute of Physiology and Nutrition, Gödöllő Campus, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary. ORCID
Balázs Kovács: Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, H-2100 Gödöllő, Hungary. ORCID
The present study aimed to adapt a Long-run Real-time DNA Damage Quantification (LORD-Q) qPCR-based method for the analysis of the mitochondrial genome of ( L.) and detect the DNA damaging effect of T-2 (4.11 mg kg) and deoxynivalenol (5.96 mg kg) mycotoxins in a 3-week feeding period. One-year-old were treated in groups (control, T-2 and DON). The mycotoxins were sprayed over the complete pelleted feed, and samples were taken weekly. Following the adaptation of LORD-Q PCR method for the species, the number of lesions were calculated to determine the amount of DNA damage. In the first and second weeks, the T-2 and the DON treated groups differed significantly from each other; however these differences disappeared in the third week. There was a significant difference in the DNA lesion values between weeks 1 and 3 in the deoxynivalenol-contaminated groups. While in the T-2 treated groups, the DNA lesion values were significantly reduced on weeks 2 and 3 compared to week 1. The results suggested that the trichothecene mycotoxins have a relevant DNA damaging effect.
