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Aug, 2021;13 (8): e17477.

Expression of Antimicrobial Peptides and Cytokines in Human Omentum Following Abdominal Surgery.

Meenu Srivastava, Abhijit Chandra, Rahul R, Jaya Nigam, Pritheesh Rajan, Devendra Parmar, Rajeshwar N Srivastava, Vivek Gupta
Author Information
  1. Meenu Srivastava: Surgical Gastroenterology, King George's Medical University, Lucknow, IND.
  2. Abhijit Chandra: Surgical Gastroenterology, King George's Medical University, Lucknow, IND.
  3. Rahul R: Surgical Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, IND.
  4. Jaya Nigam: Surgical Gastroenterology, King George's Medical University, Lucknow, IND.
  5. Pritheesh Rajan: Surgical Gastroenterology, King George's Medical University, Lucknow, IND.
  6. Devendra Parmar: Developmental Toxicology, Indian Institute of Toxicology Research, Lucknow, IND.
  7. Rajeshwar N Srivastava: Orthopaedic Surgery, King George's Medical University, Lucknow, IND.
  8. Vivek Gupta: Surgical Gastroenterology, King George's Medical University, Lucknow, IND.
PMID: 34589365 DOI: 10.7759/cureus.17477

Abstract

Introduction Omentum can secrete out biological agents like different growth factors, cytokines, and antimicrobial peptides. The aim of our study was to determine the expression of antimicrobial peptides and cytokines in human omentum tissue and its response to intra-abdominal infection. Methodology Omentum tissue was obtained from 60 patients: control (n=20) and cases (n=40). mRNA expression of antimicrobial peptides (LL-37, HBD-1, HBD-2, HNP1-3) and cytokines (TNF- α, IL-8, IL-10, IL1β) was evaluated using Real-Time PCR. Protein quantification was done by Immunoblotting and ELISA. Results Significantly higher expression of antimicrobial peptides (LL-37, HBD-1, HBD-2, HNP1-3) and cytokines (TNF- α, IL-8, IL-10, IL1β) was observed in cases as compared to control at both the transcriptional and translational level (p<0.0001). Conclusion Omentum governs a population of antimicrobial peptides with potent immunologic functions. The expression of antimicrobial peptides and cytokines is inducible and increases with the severity of infection. Omentum is thus an immunologically active and adaptable organ but its complete regulatory mechanism is still elusive.

Keywords

abdominal surgery antimicrobial peptide cytokines immunoblotting omentum rt-pcr

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