Infection in Wild Boar (): Histopathology Analysis Suggests Containment of the Infection.
Claudio Pigoli, Vito Tranquillo, Lucia Rita Gibelli, Alessandra Gaffuri, Giovanni Loris Alborali, Maria Pacciarini, Mariagrazia Zanoni, Maria Beatrice Boniotti, Giuseppe Sironi, Mario Caniatti, Valeria Grieco
Author Information
Claudio Pigoli: Laboratorio di Istologia, Sede Territoriale di Milano, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Milan, Italy.
Vito Tranquillo: Sede Territoriale di Bergamo, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Bergamo, Italy.
Lucia Rita Gibelli: Laboratorio di Istologia, Sede Territoriale di Milano, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Milan, Italy.
Alessandra Gaffuri: Sede Territoriale di Bergamo, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Bergamo, Italy.
Giovanni Loris Alborali: Sede Territoriale di Brescia, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia, Italy.
Maria Pacciarini: Dipartimento Tutela e Salute Animale, Centro di Referenza Nazionale per la Tubercolosi da Mycobacterium bovis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia, Italy.
Mariagrazia Zanoni: Sede Territoriale di Brescia, Dipartimento Area Territoriale Lombardia, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia, Italy.
Maria Beatrice Boniotti: Dipartimento Tutela e Salute Animale, Centro di Referenza Nazionale per la Tubercolosi da Mycobacterium bovis, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna, Brescia, Italy.
Giuseppe Sironi: Department of Veterinary Medicine (DIMEVET), University of Milan, Lodi, Italy.
Mario Caniatti: Department of Veterinary Medicine (DIMEVET), University of Milan, Lodi, Italy.
Valeria Grieco: Department of Veterinary Medicine (DIMEVET), University of Milan, Lodi, Italy.
The European wild boar (WB) () population has rapidly expanded over the years, raising public health concerns over the species reservoir of several pathogens, including (), a complex member. In this study, we aimed to investigate the natural infection in WB in Lombardy and Emilia Romagna Italian regions by statistically evaluating the granulomatous lesions' histological features and microbiological isolation. We analyzed 103 WB retropharyngeal and submandibular lymph nodes (LNs) for identified by PCR-restriction fragment length polymorphism, and were retrospectively selected and histologically assessed. For each sample, Hematoxylin-eosin and Ziehl-Neelsen stained slides were evaluated. Considered histological variables were: the number of granulomas, size and maturational stage of granulomas, granulomas completeness within the section, number of multinucleated giant macrophages (MGMs), and acid-fast (AF) bacilli per granuloma. Furthermore, microbiological results were also considered. microbiological isolation was negatively influenced by granulomas maturation and positively affected by AF bacilli's presence within the section. Granuloma maturation was positively influenced by granuloma size and granuloma incompleteness and negatively affected by the number of granulomas in the section and the number of MGMs within the granuloma. The results indicate that granuloma maturation should ensures an efficient containment of infection in the WB, suggesting that the intra-species transmission of the disease might be an unlikely event.
