This study aims to determine the antibiofilm effect of cell-free supernatant (CFS) of strains against strains. To study the antibiofilm mechanism against strains, antibacterial effects, cell surface properties (auto-aggregation and cell surface hydrophobicity), exopolysaccharide (EPS) production, and morphological changes were examined. The antibiofilm effect of KCCM 202399 CFS as morphological changes were evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), compared with the control treatment. Among the strains, KCCM 202399 showed the highest antibiofilm effect on KCTC 5458. The antibacterial effect of KCCM 202399 against KCTC 5458 was investigated using the deferred method (16.00 mm). The minimum inhibitory concentration of KCCM 202399 against KCTC 5458 was 25.00%. Compared with the control treatment, KCCM 202399 CFS inhibited the bacterial adhesion of KCTC 5458 by decreasing auto-aggregation, cell surface hydrophobicity, and EPS production (45.91%, 40.51%, and 67.44%, respectively). KCCM 202399 CFS inhibited and eradicated the KCTC 5458 biofilm. Therefore, these results suggest that KCCM 202399 CFS may be used to develop oral health in the probiotic industry.
