Efficacy of Cathelicidin LL-37 in an MRSA Wound Infection Mouse Model.
Oriana Simonetti, Oscar Cirioni, Gaia Goteri, Guendalina Lucarini, Elżbieta Kamysz, Wojciech Kamysz, Fiorenza Orlando, Giulio Rizzetto, Elisa Molinelli, Gianluca Morroni, Roberto Ghiselli, Mauro Provinciali, Andrea Giacometti, Annamaria Offidani
Author Information
Oriana Simonetti: Clinic of Dermatology, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60020 Ancona, Italy.
Oscar Cirioni: Clinic of Infectious Diseases, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60020 Ancona, Italy.
Gaia Goteri: Division of Pathological Anatomy, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60020 Ancona, Italy. ORCID
Guendalina Lucarini: Department of Clinical and Molecular Sciences-Histology, Polytechnic University of Marche, 60020 Ancona, Italy.
Elżbieta Kamysz: Team of Chemistry of Biological Macromolecules, Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdańsk, 80-308 Gdańsk, Poland. ORCID
Wojciech Kamysz: Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland. ORCID
Fiorenza Orlando: Experimental Animal Models for Aging Unit, Scientific Technological Area, IRCCS INRCA, 60100 Ancona, Italy. ORCID
Giulio Rizzetto: Clinic of Dermatology, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60020 Ancona, Italy. ORCID
Elisa Molinelli: Clinic of Dermatology, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60020 Ancona, Italy.
Gianluca Morroni: Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60020 Ancona, Italy.
Roberto Ghiselli: Surgical Clinic, Department of Experimental and Clinical Medicine, Polytechnic University of Marche, 60020 Ancona, Italy.
Mauro Provinciali: Experimental Animal Models for Aging Unit, Scientific Technological Area, IRCCS INRCA, 60100 Ancona, Italy. ORCID
Andrea Giacometti: Clinic of Infectious Diseases, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60020 Ancona, Italy.
Annamaria Offidani: Clinic of Dermatology, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60020 Ancona, Italy.
BACKGROUND: LL-37 is the only human antimicrobial peptide that belongs to the cathelicidins. The aim of the study was to evaluate the efficacy of LL-37 in the management of MRSA-infected surgical wounds in mice. METHODS: A wound on the back of adult male BALB/cmice was made and inoculated with . Two control groups were formed (uninfected and not treated, C0; infected and not treated, C1) and six contaminated groups were treated, respectively, with: teicoplanin, LL-37, given topically and /or systemically. Histological examination of VEGF expression and micro-vessel density, and bacterial cultures of wound tissues, were performed. RESULTS: Histological examination of wounds in the group treated with topical and intraperitoneal LL-37 showed increased re-epithelialization, formation of the granulation tissue, collagen organization, and angiogenesis. CONCLUSIONS: Based on the mode of action, LL-37 has a potential future role in the management of infected wounds.
