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Evidence-based complementary and alternative medicine : eCAM
2021;2021 4852406.

YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1.

Luandie Ge, Yaqi Fan, Lin Fu, Mengjiao Guo, Panxia Cao, Chaojie Peng, Linke Wu, Lihua Han, Hong Wu
Author Information
  1. Luandie Ge: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  2. Yaqi Fan: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  3. Lin Fu: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  4. Mengjiao Guo: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  5. Panxia Cao: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  6. Chaojie Peng: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  7. Linke Wu: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
  8. Lihua Han: Institute of Cardiovascular Disease, Henan University of Chinese Medicine, Zhengzhou 450002, China. ORCID
  9. Hong Wu: Graduate School, Henan University of Chinese Medicine, Zhengzhou 450046, China. ORCID
PMID: 34765002 DOI: 10.1155/2021/4852406

Abstract

Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher ( < 0.01) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment ( < 0.01 or < 0.05). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment ( < 0.01). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.

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