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Biological trace element research
Feb, 2022;200 (2): 535-542.

Association of TRPM5 Asn235Ser Polymorphism and Trace Elements/Minerals in Chronic Gastritis Patients: a Case-Control Study.

G Koc, A Soyocak, D Duzgun Ergun, N Pastaci Ozsobaci, S Andac-Ozturk, S Ergun
Author Information
  1. G Koc: Department of Medical Biology, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey. gulsahkoc@aydin.edu.tr. ORCID
  2. A Soyocak: Department of Medical Biology, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey. ORCID
  3. D Duzgun Ergun: Department of Biophysics, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey. ORCID
  4. N Pastaci Ozsobaci: Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey. ORCID
  5. S Andac-Ozturk: Department of Nutrition and Dietetic, Health Science Faculty, Istanbul Zaim University, Istanbul, Turkey. ORCID
  6. S Ergun: Department of General Surgery, Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey. ORCID
PMID: 34767145 DOI: 10.1007/s12011-021-03002-8

Abstract

The link between chronic gastritis and chemosensory receptors is considered promising for disease prediction and treatment. The transient receptor potential melastatin member channel 5 (TRPM5) is an ion channel and may be a chemosensor in the gastrointestinal tract. Trace elements and minerals involved in many protein structures can change ion channel activity. Our study aimed to determine trace element and mineral levels according to TRPM5 Asn235Ser missense polymorphism distributions in patients with H. pylori (+) and (-) gastritis and to uncover their possible association with disease pathogenesis. In 109 volunteer patients diagnosed with gastritis [64 H. pylori (+), 45 H. pylori (-)], TRPM5 (Asn235Ser) polymorphism was detected by Kompetitive Allele-Specific PCR method (KASP), but no difference was found between the groups. There are differences between the serum trace element (Se, Zn, Cu, Mg, and Cu/Zn) levels of the two groups. High serum Se and Cu/Zn ratios were detected in H. pylori (+) patients. Cu/Zn ratio can be used as a marker of bacterial inflammation. The amount of Se was found to be statistically significant in the serums of H. pylori (+) patients carrying TT and CT genotype, while the amounts of Zn, Cu, and Mg were found as significant of H. pylori (-) patients. The relationship of Se with the H. pylori needs to be investigated further. In addition, H. pylori (+) patients have a significantly higher neutrophils/lymphocytes ratio (NLR) compared to those with H. pylori (-) NLR can be used as an indicator of inflammation.

Keywords

Chronic gastritis Cu/Zn ratio Polymorphism Selenium TRPM5

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MeSH Term

Case-Control Studies
Gastritis
Helicobacter pylori
Humans
Minerals
TRPM Cation Channels
Trace Elements

Chemicals

Minerals
TRPM Cation Channels
TRPM5 protein, human
Trace Elements
Journal Article

Word Cloud

Created with Highcharts 10.0.0HpyloripatientsTRPM5+gastritis-SeCu/ZnchannelcanAsn235SerfoundratiodiseaseionTracetraceelementlevelspolymorphismdetectedgroupsserumZnCuMgusedinflammationsignificantNLRPolymorphismChroniclinkchronicchemosensoryreceptorsconsideredpromisingpredictiontreatmenttransientreceptorpotentialmelastatinmember5maychemosensorgastrointestinaltractelementsmineralsinvolvedmanyproteinstructureschangeactivitystudyaimeddeterminemineralaccordingmissensedistributionsuncoverpossibleassociationpathogenesis109volunteerdiagnosed[6445]KompetitiveAllele-SpecificPCRmethodKASPdifferencedifferencestwoHighratiosmarkerbacterialamountstatisticallyserumscarryingTTCTgenotypeamountsrelationshipneedsinvestigatedadditionsignificantlyhigherneutrophils/lymphocytescomparedindicatorAssociationElements/MineralsGastritisPatients:Case-ControlStudySelenium

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