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Foods (Basel, Switzerland)
Nov 09, 2021;10 (11):

The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico.

Liza Devita, Hanifah Nuryani Lioe, Mala Nurilmala, Maggy T Suhartono
Author Information
  1. Liza Devita: Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Bogor 16680, Indonesia.
  2. Hanifah Nuryani Lioe: Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Bogor 16680, Indonesia. ORCID
  3. Mala Nurilmala: Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor 16680, Indonesia.
  4. Maggy T Suhartono: Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Bogor 16680, Indonesia.
PMID: 34829019 DOI: 10.3390/foods10112739

Abstract

The hydrolysates and peptide fractions of bigeye tuna () skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17-135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

Keywords

Thunnus obesus antioxidant activity bioactive peptides collagen protein enzymatic hydrolysis fishery by-product in silico ultrafiltration peptide fractions

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Grants

  1. /The Ministry of Agriculture Republic Indonesia
Journal Article

Word Cloud

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