Phenolic Analysis and In Vitro Biological Activity of Red Wine, Pomace and Grape Seeds Oil Derived from L. cv. Montepulciano d'Abruzzo.
Adriano Mollica, Giuseppe Scioli, Alice Della Valle, Angelo Cichelli, Ettore Novellino, Marta Bauer, Wojciech Kamysz, Eulogio J Llorent-Martínez, Maria Luisa Fernández-de Córdova, R Castillo-López, Gunes Ak, Gokhan Zengin, Stefano Pieretti, Azzurra Stefanucci
Author Information
Adriano Mollica: Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy. ORCID
Giuseppe Scioli: Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Alice Della Valle: Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Angelo Cichelli: Department of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Ettore Novellino: NGN Healthcare, Via Nazionale Torrette, 207, 83013 Mercogliano, Italy.
Marta Bauer: Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdànsk, 80-416 Gdànsk, Poland.
Wojciech Kamysz: Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdànsk, 80-416 Gdànsk, Poland.
Eulogio J Llorent-Martínez: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, E-23071 Jaén, Spain. ORCID
Maria Luisa Fernández-de Córdova: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, E-23071 Jaén, Spain. ORCID
R Castillo-López: Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, E-23071 Jaén, Spain.
Gunes Ak: Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey.
Gokhan Zengin: Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey. ORCID
Stefano Pieretti: National Center for Drug Research and Evaluation, Italian National Institute of Health, Viale Regina Elena 299, 00161 Rome, Italy. ORCID
Azzurra Stefanucci: Department of Pharmacy, G. d'Annunzio University of Chieti-Pescara, 66100 Chieti, Italy.
Grape pomace is commonly considered a waste product of monovarietal red wine production. Methods: HPLC-DAD analysis was performed to determine the polyphenol and flavonoid contents of all the extracts obtained from Montepulciano d'Abruzzo red wine and grape skins whereas, GC-MS was applied to the determination of fatty acid composition in grape seeds oil. Biological characterization involves antioxidant and antimicrobial assays for all the extracts and seeds oil; Their ability to inhibit α-glucosidase, α-amylase, α-tyrosinase, and ChE enzymes was also detected, together with anti-inflammatory activity on wine, grape skin extracts, and seeds oil by lipoxygenase (5-LOX) and LPS-stimulated macrophage release assays. Data indicate significative polyphenols content (199.31 ± 7.21 mgGAE/g), antioxidant (CUPRAC assay (1036.98 mgTE/g)), enzymatic inhibition (α-tyrosinase: 151.30 ± 1.20 mgKAE/g) and anti-inflammatory activities for wine-organic extract 2, while the antimicrobial activity of grape skin decoction is higher than those reported by wine extracts on three bacterial strains. Interestingly only dealcoholized wine and wine-aqueous extract exerts inhibitory effects on α-glucosidase (20.62 ± 0.23 mmolACAE/g and 19.81 ± 0.03 mmolACAE/g, respectively), while seeds oil is rich in oleic and linoleic acids. These results confirm the strong antioxidant properties of Montepulciano d'Abruzzo grape pomace, suggesting the potential use of this waste product as functional food supplements in the human diet and in cosmeceutics.
