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2021;16 (11): e0260103.

H19- and hsa-miR-338-3p-mediated NRP1 expression is an independent predictor of poor prognosis in glioblastoma.

Yong Liu, Yuelin Liu, Yong Gao, Lei Wang, Hengliang Shi, Chengmin Xuan
Author Information
  1. Yong Liu: Department of Neurosurgery, Brain Hospital, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  2. Yuelin Liu: Department of Clinical Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  3. Yong Gao: Department of Orthopaedics, Xuzhou Children's Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  4. Lei Wang: Department of Neurosurgery, Brain Hospital, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  5. Hengliang Shi: Department of Clinical Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China.
  6. Chengmin Xuan: Medical Section, Xuzhou Children's Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, P.R. China. ORCID
PMID: 34843522 DOI: 10.1371/journal.pone.0260103

Abstract

Glioblastoma multiforme (GBM) is the most common and also the most invasive brain cancer. GBM progression is rapid and its prognosis is poor. Therefore, finding molecular targets in GBM is a critical goal that could also play important roles in clinical diagnostics and treatments to improve patient prognosis. We jointly analyzed the GSE103227, GSE103229, and TCGA databases for differentially expressed RNA species, obtaining 52 long non-coding RNAs (lncRNAs), 31 microRNAs (miRNAs), and 186 mRNAs, which were used to build a competing endogenous RNA network. Kaplan-Meier and receiver operating characteristic (ROC) analyses revealed five survival-related lncRNAs: H19, LINC01574, LINC01614, RNF144A-AS1, and OSMR-AS1. With multiple optimization mRNAs, we found the H19-hsa-miR-338-3P-NRP1 regulatory pathway. Additionally, we noted high NRP1 expression in GBM patients, and Kaplan-Meier and ROC analyses showed that NRP1 expression was associated with GBM prognosis. Cox analysis indicated that NRP1 is an independent prognostic factor in GBM patients. In conclusion, H19 and hsa-miR-338-3P regulate NRP1 expression, and this pathway plays an important role in GBM.

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MeSH Term

Brain
Brain Neoplasms
Databases, Genetic
Glioblastoma
Humans
Kaplan-Meier Estimate
MicroRNAs
Neuropilin-1
Prognosis
RNA, Long Noncoding
ROC Curve

Chemicals

H19 long non-coding RNA
MIRN338 microRNA, human
MicroRNAs
NRP1 protein, human
RNA, Long Noncoding
Neuropilin-1
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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