Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of and Their Impact on Bacterial Physiology and Pathogenesis.
Anna M Banaś, Katarzyna M Bocian-Ostrzycka, Stanisław Dunin-Horkawicz, Jan Ludwiczak, Piotr Wilk, Marta Orlikowska, Agnieszka Wyszyńska, Maria Dąbrowska, Maciej Plichta, Marta Spodzieja, Marta A Polańska, Agata Malinowska, Elżbieta Katarzyna Jagusztyn-Krynicka
Author Information
Anna M Banaś: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, 02-096 Warsaw, Poland. ORCID
Katarzyna M Bocian-Ostrzycka: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, 02-096 Warsaw, Poland.
Stanisław Dunin-Horkawicz: Laboratory of Structural Bioinformatics, Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland.
Jan Ludwiczak: Laboratory of Structural Bioinformatics, Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland. ORCID
Piotr Wilk: Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Cracow, Poland. ORCID
Marta Orlikowska: Faculty of Chemistry, University of Gdańsk, 80-308 Gdansk, Poland.
Agnieszka Wyszyńska: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, 02-096 Warsaw, Poland. ORCID
Maria Dąbrowska: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, 02-096 Warsaw, Poland. ORCID
Maciej Plichta: Laboratory of Biological Chemistry of Metal Ions, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland. ORCID
Marta Spodzieja: Faculty of Chemistry, University of Gdańsk, 80-308 Gdansk, Poland. ORCID
Marta A Polańska: Department of Animal Physiology, Faculty of Biology, Institute of Functional Biology and Ecology, University of Warsaw, 02-096 Warsaw, Poland.
Agata Malinowska: Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland. ORCID
Elżbieta Katarzyna Jagusztyn-Krynicka: Department of Bacterial Genetics, Faculty of Biology, Institute of Microbiology, University of Warsaw, 02-096 Warsaw, Poland. ORCID
The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here, we report the detailed analysis of the Dsb pathway of . The oxidizing Dsb system of this pathogen is unique because it consists of two monomeric DsbAs (DsbA1 and DsbA2) and one dimeric bifunctional protein (C8J_1298). Previously, we showed that DsbA1 and C8J_1298 are redundant. Here, we unraveled the interaction between the two monomeric DsbAs by in vitro and in vivo experiments and by solving their structures and found that both monomeric DsbAs are dispensable proteins. Their structures confirmed that they are homologs of EcDsbL. The slight differences seen in the surface charge of the proteins do not affect the interaction with their redox partner. Comparative proteomics showed that several respiratory proteins, as well as periplasmic transport proteins, are targets of the Dsb system. Some of these, both donors and electron acceptors, are essential elements of the respiratory process under oxygen-limiting conditions in the host intestine. The data presented provide detailed information on the function of the Dsb system, identifying it as a potential target for novel antibacterial molecules.
