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Frontiers in endocrinology
2021;12 802768.

Evidences for Red Pigment Concentrating Hormone () and Beta-Pigment Dispersing Hormone () Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, .

Ling-Ling Wei, Tian-Tian Chen, Bi-Yun Luo, Gao-Feng Qiu
Author Information
  1. Ling-Ling Wei: National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
  2. Tian-Tian Chen: National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
  3. Bi-Yun Luo: National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
  4. Gao-Feng Qiu: National Demonstration Center for Experimental Fisheries Science Education, Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
PMID: 34975771 DOI: 10.3389/fendo.2021.802768

Abstract

Red pigment concentrating hormone (RPCH) and pigment dispersing hormone (PDH) are crustacean neuropeptides involved in broad physiological processes including body color changes, circadian rhythm, and ovarian growth. In this study, the full-length cDNA of and were identified from the brain of the Chinese mitten crab . The deduced RPCH and PDH mature peptides shared identical sequence to the adipokinetic hormone/RPCH peptides family and the β-PDH isoforms and were designated as Es-RPCH and Es-β-PDH, respectively. and transcripts were distributed in the brain and eyestalks. The positive signals of and were localized in the neuronal clusters 6, 8, 9, 10, and 17 of the brain as revealed by hybridization. The expression level of and mRNA in nervous tissues were all significantly increased at vitellogenic stage, and then decreased at the final meiotic maturation stage. The administrated with synthesized Es-RPCH peptide results in germinal vesicles shift toward the plasma membrane in vitellogenic oocyte, and significant decrease of the gonad-somatic index (GSI) and mean oocyte diameter as well as the expression of vitellogenin mRNA at 30 days post injection . Similar results were also found when injection of the Es-β-PDH peptide. culture demonstrated that Es-RPCH and Es-β-PDH induced germinal vesicle breakdown of the late vitellogenic oocytes. Comparative ovarian transcriptome analysis indicated that some reproduction/meiosis-related genes such as cdc2 kinase, cyclin B, 5-HT-R and retinoid-X receptor were significantly upregulated in response to Es-RPCH and Es-β-PDH treatments. Taken together, these results provided the evidence for the inductive effect of and on the oocyte meiotic maturation in .

Keywords

Eriocheir sinensis ISH PDH RPCH oocyte meiosis transcriptome

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MeSH Term

Animals
Brachyura
Brain Chemistry
China
DNA, Complementary
Female
Gene Expression
Meiosis
Oligopeptides
Oocytes
Ovary
Peptides
Pyrrolidonecarboxylic Acid
RNA, Messenger
Vitellogenesis

Chemicals

DNA, Complementary
Oligopeptides
Peptides
RNA, Messenger
red pigment-concentrating hormone
melanophore-dispersing hormone
Pyrrolidonecarboxylic Acid
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 3

Word Cloud

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