Fabrication of 3D Amino-Functionalized Metal-Organic Framework on Porous Nickel Foam Skeleton to Combinate Follicle Stimulating Hormone Antibody for Specific Recognition of Follicle-Stimulating Hormone.
Sathyadevi Palanisamy, Hsu-Min Wu, Li-Yun Lee, Shyng-Shiou F Yuan, Yun-Ming Wang
Author Information
Sathyadevi Palanisamy: Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDSB), National Yang Ming Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, Taiwan.
Hsu-Min Wu: Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDSB), National Yang Ming Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, Taiwan.
Li-Yun Lee: Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDSB), National Yang Ming Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, Taiwan.
Shyng-Shiou F Yuan: Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, Taiwan. ORCID
Yun-Ming Wang: Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDSB), National Yang Ming Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, Taiwan. ORCID
In this study, a superficial and highly efficient hydrothermal synthesis method was developed for the in situ growth of amine-functionalized iron containing metal-organic frameworks (HN-Fe-MIL-101 MOFs) on porous nickel foam (NicF) skeletons (HN-Fe-MIL-101/NicF). The uniform decoration of the HN-Fe-MIL-101 nanosheets thus generated on NicF was immobilized with follicle-stimulating hormone (FSH) antibody (Ab-FSH) to detect FSH antigen. In the present work, the Ab-FSH tagged HN-Fe-MIL-101/NicF electrode was first applied as an immunosensor for the recognition of FSH, electrochemically. With all of the special characteristics, this material demonstrated superior specific recognition and sensitivity for FSH with an estimated detection limit (LOD) of 11.6 and 11.5 fg/mL for buffered and serum solutions, respectively. The availability of specific functional groups on MOFs makes them an interesting choice for exploring molecular sensing applications utilizing Ab-FSH tagged biomolecules.
