Chemical Profiling, Bioactivity Evaluation and the Discovery of a Novel Biopigment Produced by CBS 113139.
Antonis D Tsiailanis, Chrysanthi Pateraki, Mary Kyriazou, Christos M Chatzigiannis, Maria Chatziathanasiadou, Nikolaos Parisis, Ioanna Mandala, Andreas G Tzakos, Apostolis Koutinas
Author Information
Antonis D Tsiailanis: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece.
Chrysanthi Pateraki: Department of Food Science and Human Nutrition, Agricultural University of Athens, 118 55 Athens, Greece. ORCID
Mary Kyriazou: Department of Food Science and Human Nutrition, Agricultural University of Athens, 118 55 Athens, Greece.
Christos M Chatzigiannis: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece.
Maria Chatziathanasiadou: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece.
Nikolaos Parisis: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece. ORCID
Ioanna Mandala: Department of Food Science and Human Nutrition, Agricultural University of Athens, 118 55 Athens, Greece.
Andreas G Tzakos: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, 451 10 Ioannina, Greece. ORCID
Apostolis Koutinas: Department of Food Science and Human Nutrition, Agricultural University of Athens, 118 55 Athens, Greece.
Biobased pigments are environmentally friendly alternatives to synthetic variants with an increased market demand. Production of pigments via fermentation is a promising process, yet optimization of the production yield and rate is crucial. Herein, we evaluated the potential of to produce biobased pigments. Optimum sugar concentration was 30 g/L and optimum C:N ratio was 36:1 resulting in the production of 4.1-4.5 AU (namely Pigment Complex A). Supplementation with ammonium nitrate resulted in the production of 4.1-4.9 AU (namely Pigment Complex B). Pigments showed excellent pH stability. The major biopigments in Pigment Complex A were or the acid form of (red pigment), (violet pigment), (orange pigment) and . In Pigment Complex B, a novel biopigment annotated as was identified. Its basic structure contains a polyketide azaphilone with the same carboxyl-monascorubramine base structure as (violet pigment) and . The pigments were not cytotoxic up to 250 μg/mL.
MIS 5031817/Operational Programme "Competitiveness, Entrepreneurship and Innovation" (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).
