Yinbo Ma: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Su Ryun Choi: Molecular Genetics and Genomics Laboratory, Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea.
Yu Wang: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Sushil Satish Chhapekar: Molecular Genetics and Genomics Laboratory, Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea.
Xue Zhang: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Yingjun Wang: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Xueying Zhang: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Meiyu Zhu: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Di Liu: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Zhennan Zuo: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Xinyu Yan: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Caixia Gan: Cash Crops Research Institute, Hubei Academy of Agricultural Sciences; Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement, Wuhan 430070, China.
Di Zhao: Analytical and Testing center, Shenyang agricultural university, Shenyang 110866, China.
Yue Liang: College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Wenxing Pang: College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Yong Pyo Lim: Molecular Genetics and Genomics Laboratory, Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea.
Clubroot is one of the major diseases adversely affecting Chinese cabbage (Brassica rapa) yield and quality. To precisely characterize the Plasmodiophora brassicae infection on Chinese cabbage, we developed a dual fluorescent staining method for simultaneously examining the pathogen, cell structures, and starch grains. The number of starch (amylopectin) grains increased in B. rapa roots infected by P. brassicae, especially from 14 to 21 days after inoculation. Therefore, the expression levels of 38 core starch metabolism genes were investigated by quantitative real-time PCR. Most genes related to starch synthesis were up-regulated at seven days after the P. brassicae inoculation, whereas the expression levels of the starch degradation-related genes increased at 14 days after the inoculation. Then genes encoding the core enzymes involved in starch metabolism were investigated by assessing their chromosomal distributions, structures, duplication events, and synteny among Brassica species. Genome comparisons indicated that 38 non-redundant genes belonging to six core gene families related to starch metabolism are highly conserved among Arabidopsis thaliana, B. rapa, Brassica nigra, and Brassica oleracea. Genome sequencing projects have revealed that P. brassicae obtained host nutrients by manipulating plant metabolism. starch may serve as a carbon source for P. brassicae colonization as indicated by the histological observation and transcriptomic analysis. Results of this study may elucidate the evolution and expression of core starch metabolism genes and provide researchers with novel insights into the pathogenesis of Clubroot in B. rapa.
References
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
[PMID: 29722887]
Front Plant Sci. 2020 Aug 31;11:568771
[PMID: 32983217]
Front Plant Sci. 2018 Sep 21;9:1344
[PMID: 30298078]
