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Dec, 2022;47 (12): 3615-3626.

Characteristics of Epileptiform Spike-wave Discharges and Chronic Histopathology in Controlled Cortical Impact Model of Sprague-Dawley Rats.

Lei Sun, Ru Liu, Huajun Yang, Tingting Yu, Jianping Wu, Qun Wang
Author Information
  1. Lei Sun: Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
  2. Ru Liu: Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
  3. Huajun Yang: Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, 100070, China.
  4. Tingting Yu: Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China.
  5. Jianping Wu: Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China. biojpwu@ccmu.edu.cn.
  6. Qun Wang: Beijing Tiantan Hospital, Capital Medical University, Beijing, 100070, China. wangq@ccmu.edu.cn.
PMID: 35103912 DOI: 10.1007/s11064-022-03542-y

Abstract

Post-traumatic epilepsy (PTE) is a serious complication that can occur following traumatic brain injury (TBI). Sustained secondary changes after TBI promote the process of PTE. Here, we aim to evaluate changes in behavior, electrocorticogram, and histomorphology in rats following chronic TBI models. We observed intensive 7-8 Hz spike-wave-discharges (SWDs) at frontal recording sites and quantified them in SD rats with different degrees of TBI and compared them with age-matched sham rats to evaluate the association between SWDs and injury severity. Notably, although SWDs were even presented in the sham group, the number and duration of events were much lower than those in the TBI groups. SWDs have numerous similarities to absence seizures, such as abrupt onset, termination, and lack of postictal suppression, which may be the nonconvulsive characteristics of PTE. Retigabine, a novel antiepileptic drug, is ineffective in reducing SWDs. In addition, we examined chronic histopathological changes in TBI rats. Rats subjected to moderate and severe TBI exhibited significantly impaired neurological function, which was accompanied by marked cortical injury, hippocampus deformation, reactive gliosis, and mossy fiber sprouting. Long-term progressive structural changes in the brain are one of the characteristics of epileptogenesis after TBI. Our study provided the potential value of epileptiform SWDs in reflecting the nonconvulsive characteristic of PTE and highlighted the vital role of chronic pathological changes, such as reactive gliosis, in promoting the epileptogenesis following TBI.

Keywords

Chronic histopathology Nonconvulsive seizure Reactive gliosis Spike-wave discharges Traumatic brain injury

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Grants

  1. 20181101/Capital Medical University Advanced Innovation Center for Human Brain Protection
  2. 2017YFC1307501/National Key R&D Program of China
  3. H2018206435/Beijing-Tianjin-Hebei Cooperative Basic Research Program
  4. 81870935/National Natural Science Foundation of China

MeSH Term

Rats
Animals
Humans
Rats, Sprague-Dawley
Gliosis
Patient Discharge
Epilepsy, Post-Traumatic
Seizures
Brain Injuries, Traumatic
Disease Models, Animal
Electroencephalography
Journal Article

Word Cloud

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