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Frontiers in genetics
2021;12 730599.

Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments.

Preeti Verma, Ankita Sharma, Monika Sodhi, Manish Tiwari, Prince Vivek, Ranjit S Kataria, S K Nirajan, Vijay K Bharti, Pawan Singh, S S Lathwal, Vishal Sharma, Nampher Masharing, Manishi Mukesh
Author Information
  1. Preeti Verma: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  2. Ankita Sharma: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  3. Monika Sodhi: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  4. Manish Tiwari: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  5. Prince Vivek: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  6. Ranjit S Kataria: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  7. S K Nirajan: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  8. Vijay K Bharti: DRDO-Defense Institute of High-altitude Research, Leh, India.
  9. Pawan Singh: Animal Biotechnology Center, ICAR-National Dairy Research Institute, Karnal, India.
  10. S S Lathwal: Animal Biotechnology Center, ICAR-National Dairy Research Institute, Karnal, India.
  11. Vishal Sharma: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  12. Nampher Masharing: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
  13. Manishi Mukesh: Animal Biotechnology Division, ICAR-National Bureau of Animal Genetic Resources, Karnal, India.
PMID: 35178065 DOI: 10.3389/fgene.2021.730599

Abstract

To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs). To date, information on an appropriate panel of RGs in cattle populations reared at cold arid high-altitude hypoxia and hot arid tropical normoxia environments is not available. Therefore, the present study was carried out to identify a panel of stably expressed RGs from 10 candidate genes ( and ) in peripheral blood mononuclear cells (PBMCs) of cattle populations reared at cold arid high-altitude hypoxia and hot arid normoxia environments. Four different statistical algorithms: geNorm, NormFinder, BestKeeper, and RefFinder were used to assess the stability of these genes. A total of 30 blood samples were collected: six adult heifers each of Ladakhi (LAC) and Holstein Frisian crosses (HFX) and 4 Jersey (JYC) cows from cold arid high-altitude hypoxia environments (group I) and five adult heifers each of Sahiwal (SAC), Karan Fries (KFC), and Holstein Friesian (HFC) cows from hot arid normoxia environments (group II). Combined analysis of group I and group II resulted in identification of a panel of RGs like and that could act as a useful resource to unravel the accurate transcriptional profile of PBMCs from diverse cattle populations adapted to distinct altitudes.

Keywords

PBMCs cattle cold arid expression stability hot arid normalization qRT-PCR reference genes

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Word Cloud

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