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Methods in molecular biology (Clifton, N.J.)
2022;2442 425-443.

Manipulating Galectin Expression in Zebrafish (Danio rerio).

Chiguang Feng, Mihai Nita-Lazar, Nuria González-Montalbán, Jingyu Wang, Justin Mancini, Sheng Wang, Chinnarajan Ravindran, Hafiz Ahmed, Gerardo R Vasta
Author Information
  1. Chiguang Feng: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  2. Mihai Nita-Lazar: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  3. Nuria González-Montalbán: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  4. Jingyu Wang: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  5. Justin Mancini: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  6. Sheng Wang: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  7. Chinnarajan Ravindran: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  8. Hafiz Ahmed: Department of Biochemistry, School of Medicine, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA.
  9. Gerardo R Vasta: Department of Microbiology and Immunology, Institute of Marine and Environmental Technology, University of Maryland Baltimore, Baltimore, MD, USA. gvasta@som.umaryland.edu.
PMID: 35320539 DOI: 10.1007/978-1-0716-2055-7_23

Abstract

Techniques for disrupting gene expression are invaluable tools for the analysis of the biological role of a gene product. Because of its genetic tractability and multiple advantages over conventional mammalian models, the zebrafish (Danio rerio) is recognized as a powerful system for gaining new insight into diverse aspects of human health and disease. Among the multiple mammalian gene families for which the zebrafish has shown promise as an invaluable model for functional studies, the galectins have attracted great interest due to their participation in early development, regulation of immune homeostasis, and recognition of microbial pathogens. Galectins are β-galactosyl-binding lectins with a characteristic sequence motif in their carbohydrate recognition domains (CRDs), that constitute an evolutionary conserved family ubiquitous in eukaryotic taxa. Galectins are emerging as key players in the modulation of many important pathological processes, which include acute and chronic inflammatory diseases, autoimmunity and cancer, thus making them potential molecular targets for innovative drug discovery. Here, we provide a review of the current methods available for the manipulation of gene expression in the zebrafish, with a focus on gene knockdown [morpholino (MO)-derived antisense oligonucleotides] and knockout (CRISPR-Cas) technologies.

Keywords

CRISPR-Cas Galectins Gene expression Microinjection Morpholino Zebrafish

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Grants

  1. R01 GM070589/NIGMS NIH HHS

MeSH Term

Animals
Galectins
Gene Knockdown Techniques
Mammals
Morpholinos
RNA
Zebrafish

Chemicals

Galectins
Morpholinos
RNA
Journal Article Review Research Support, N.I.H., Extramural

Word Cloud

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