Inanimate Surfaces and Air Contamination with Multidrug Resistant Species of in the Neonatal Intensive Care Unit Environment.
Ralciane de Paula Menezes, Lara de Andrade Marques, Felipe Flávio Silva, Nagela Bernadelli Sousa Silva, Priscila Guerino Vilela Alves, Meliza Arantes de Souza Bessa, Lúcio Borges de Araújo, Mário Paulo Amante Penatti, Reginaldo Dos Santos Pedroso, Denise Von Dolinger de Brito Röder
Author Information
Ralciane de Paula Menezes: Technical Course in Clinical Analysis, Technical School of Health (ESTES), Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
Lara de Andrade Marques: Postgraduate Program in Health Sciences, Faculty of Medicine, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
Felipe Flávio Silva: Postgraduate Program in Health Sciences, Faculty of Medicine, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
Nagela Bernadelli Sousa Silva: Postgraduate Program in Health Sciences, Faculty of Medicine, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
Priscila Guerino Vilela Alves: Postgraduate Program in Health Sciences, Faculty of Medicine, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil. ORCID
Meliza Arantes de Souza Bessa: Institute of Biology, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil. ORCID
Lúcio Borges de Araújo: Faculty of Medicine, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil. ORCID
Mário Paulo Amante Penatti: Technical Course in Clinical Analysis, Technical School of Health (ESTES), Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
Reginaldo Dos Santos Pedroso: Technical Course in Clinical Analysis, Technical School of Health (ESTES), Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil. ORCID
Denise Von Dolinger de Brito Röder: Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia 38400-320, MG, Brazil.
BACKGROUND: Contamination of the hospital environment with multi-resistant (MDR) increases the risk of infection. The aim of this study is to identify the MDR species of on inanimate surfaces, in air, and in clinical samples, and analyze the risk factors that correlate with the occurrence of infections in a Neonatal Intensive Care Unit. METHODS: Samples of inanimate surfaces and air were taken using a premoistened swab (0.9% sodium chloride) and spontaneous air sedimentation, respectively. The clinical isolates were recovered from infected neonates. The isolates (environmental and clinical) were identified by matrix-assisted laser desorption ionization-time of flight and the resistance profile was calculated using the disk diffusion agar technique. RESULTS: In total, 181 isolates were obtained, 93 from (surfaces), 18 from the air, and 70 clinical samples. was the most frequent species (66.8%), and the failure rate in air cleaning was 100%. More than 60% of the isolates were MDR, and the majority of clinical isolates (60.4%) had a resistance profile identical to that of the environmental isolates. CONCLUSION: spp. were found in most of the analyzed samples, with a high frequency of MDR isolates, demonstrating the importance of the hospital environment as a reservoir, and the need for infection control measures, and rational use of antimicrobials.
