OpenLB
Open Library of Bioscience
Advanced Search
Frontiers in public health
2022;10 787299.

qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment.

Jingheng Zhang, Fang Yu, Keyun Fu, Xinyu Ma, Yi Han, Chi Ching Ali, Haonan Zhou, Yantao Xu, Tingyue Zhang, Shuntong Kang, Yiming Xu, Zhuolin Li, Jiaqi Shi, Shuai Gao, Yongyi Chen, Liyu Chen, Jianglin Zhang, Feizhou Zhu
Author Information
  1. Jingheng Zhang: Xiangya School of Medicine, Central South University, Changsha, China.
  2. Fang Yu: Xiangya School of Medicine, Central South University, Changsha, China.
  3. Keyun Fu: Xiangya School of Medicine, Central South University, Changsha, China.
  4. Xinyu Ma: Xiangya School of Medicine, Central South University, Changsha, China.
  5. Yi Han: Xiangya School of Medicine, Central South University, Changsha, China.
  6. Chi Ching Ali: Xiangya School of Medicine, Central South University, Changsha, China.
  7. Haonan Zhou: Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, China.
  8. Yantao Xu: Xiangya School of Medicine, Central South University, Changsha, China.
  9. Tingyue Zhang: Xiangya School of Medicine, Central South University, Changsha, China.
  10. Shuntong Kang: Xiangya School of Medicine, Central South University, Changsha, China.
  11. Yiming Xu: Xiangya School of Medicine, Central South University, Changsha, China.
  12. Zhuolin Li: Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, China.
  13. Jiaqi Shi: Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, China.
  14. Shuai Gao: Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, China.
  15. Yongyi Chen: Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
  16. Liyu Chen: Department of Microbiology, School of Basic Medical Sciences, Central South University, Changsha, China.
  17. Jianglin Zhang: Department of Dermatology, Shenzhen People's Hospital, Shenzhen, China.
  18. Feizhou Zhu: Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, Changsha, China.
PMID: 35372231 DOI: 10.3389/fpubh.2022.787299

Abstract

Background: Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide misuse a frequent occurrence around the globe, with serious consequences.
Objective: We developed (qPCR)-based antibiotics resistance assay (ACQUIRE) to enable fast and accurate detection of macrolide resistance in clinical settings, representing an opportunity to administer antibiotics more wisely and improve the quality of care.
Methods: A cross-sectional observational study ( = 915) was conducted to probe into the macrolide resistance of in patients with acne.
Results: The high sensitivity of ACQUIRE enabled us to reveal a much higher 23S recombinant DNA (rDNA) point mutation rate (52%) and thus a higher macrolide resistance (75.5%) compared to previous reports. Carriage of ermX gene was discovered on 472 (53%) subjects, which concurs with previous studies.
Conclusion: The macrolide resistance of is much higher than previously reported. Integrating ACQUIRE into acne treatment modalities may eliminate macrolide misuse and achieve better clinical improvements.

Keywords

acne (acne vulgaris) antimicrobial antimicrobial resistance macrolide-resistant gene quantitative PCR (qPCR)

References

  1. J Invest Dermatol. 2013 Sep;133(9):2152-60 [PMID: 23337890]
  2. BMJ Open. 2019 Feb 19;9(2):e022938 [PMID: 30782869]
  3. Br J Dermatol. 2018 Jul;179(1):200-201 [PMID: 29368337]
  4. Australas J Dermatol. 2012 May;53(2):106-11 [PMID: 22571557]
  5. J Eur Acad Dermatol Venereol. 2017 Sep;31 Suppl 5:8-12 [PMID: 28805938]
  6. J Antimicrob Chemother. 2002 Jan;49(1):165-8 [PMID: 11751782]
  7. Br J Dermatol. 2003 Mar;148(3):467-78 [PMID: 12653738]
  8. Sci Rep. 2016 Dec 21;6:39491 [PMID: 28000755]
  9. J Am Acad Dermatol. 2009 May;60(5 Suppl):S1-50 [PMID: 19376456]
  10. J Am Acad Dermatol. 2019 Feb;80(2):538-549 [PMID: 30296534]
  11. Lancet Infect Dis. 2016 Mar;16(3):e23-33 [PMID: 26852728]
  12. Nat Rev Dis Primers. 2015 Sep 17;1:15029 [PMID: 27189872]
  13. Dermatology. 1998;196(1):69-70 [PMID: 9557230]
  14. J Dermatol. 2017 Nov;44(11):1248-1254 [PMID: 28623856]
  15. Int J Dermatol. 2013 Apr;52(4):418-25 [PMID: 23432195]
  16. J Dermatol. 2016 Apr;43(4):406-13 [PMID: 26508505]
  17. J Am Acad Dermatol. 2006 Oct;55(4):691-2 [PMID: 17010751]
  18. J Am Acad Dermatol. 2016 Feb;74(2):273-9 [PMID: 26525749]
  19. J Am Acad Dermatol. 2020 May;82(5):1268-1269 [PMID: 32303330]
  20. Br J Dermatol. 2001 Feb;144(2):339-46 [PMID: 11251569]
  21. Int J Syst Evol Microbiol. 2016 Nov;66(11):4422-4432 [PMID: 27488827]
  22. N Engl J Med. 2018 Oct 04;379(14):1343-1352 [PMID: 30281982]
  23. Int J Dermatol. 2013 Jun;52(6):688-92 [PMID: 23451873]
  24. J Am Acad Dermatol. 2016 May;74(5):945-73.e33 [PMID: 26897386]
  25. Lancet. 2012 Jan 28;379(9813):361-72 [PMID: 21880356]
  26. J Eur Acad Dermatol Venereol. 2013 Dec;27(12):1546-51 [PMID: 23279041]
  27. Int J Dermatol. 2016 Nov;55(11):1196-1204 [PMID: 27421121]

MeSH Term

Acne Vulgaris
Anti-Bacterial Agents
Cross-Sectional Studies
Drug Resistance, Bacterial
Humans
Macrolides
Microbial Sensitivity Tests

Chemicals

Anti-Bacterial Agents
Macrolides
Journal Article Observational Study Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

Created with Highcharts 10.0.0resistancemacrolideacneACQUIREhighermisuseqPCRantibioticsclinicalmuchpreviousgeneResistanceMacrolideAcneantimicrobialBackground:Macrolideswidelyusedtreatmoderate-to-severe50yearsHoweverprevalentantibioticPropionibacteriumacnesalongabsenceclinicallyavailabletestsmadefrequentoccurrencearoundglobeseriousconsequencesObjective:developed-basedassayenablefastaccuratedetectionsettingsrepresentingopportunityadministerwiselyimprovequalitycareMethods:cross-sectionalobservationalstudy=915conductedprobepatientsResults:highsensitivityenabledusreveal23SrecombinantDNArDNApointmutationrate52%thus755%comparedreportsCarriageermXdiscovered47253%subjectsconcursstudiesConclusion:previouslyreportedIntegratingtreatmentmodalitiesmayeliminateachievebetterimprovementsqPCR-BasedAntibioticsAssayRevealsWidespreadPatientsCanEliminateMisuseTreatmentvulgarismacrolide-resistantquantitativePCR

Similar Articles

Cited By