Metabolic Profiling of Interspecies Interactions During Sessile Bacterial Cultivation Reveals Growth and Sporulation Induction in in Response to .
Jakob Herschend, Madeleine Ernst, Klaus Koren, Alexey V Melnik, Ricardo R da Silva, Henriette L Røder, Zacharias B V Damholt, Per Hägglund, Birte Svensson, Søren J Sørensen, Michael Kühl, Pieter C Dorrestein, Mette Burmølle
Author Information
Jakob Herschend: Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Madeleine Ernst: Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, United States.
Klaus Koren: Aarhus University Centre for Water Technology (WATEC), Department of Biology, Aarhus University, Aarhus, Denmark.
Alexey V Melnik: Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, United States.
Ricardo R da Silva: Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, United States.
Henriette L Røder: Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Zacharias B V Damholt: Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark.
Per Hägglund: Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark.
Birte Svensson: Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs Lyngby, Denmark.
Søren J Sørensen: Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Michael Kühl: Marine Biological Section, Department of Biology, University of Copenhagen, Helsingør, Denmark.
Pieter C Dorrestein: Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA, United States.
Mette Burmølle: Section of Microbiology, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
The toolbox available for microbiologists to study interspecies interactions is rapidly growing, and with continuously more advanced instruments, we are able to expand our knowledge on establishment and function of microbial communities. However, unravelling molecular interspecies interactions in complex biological systems remains a challenge, and interactions are therefore often studied in simplified communities. Here we perform an in-depth characterization of an observed interspecies interaction between two co-isolated bacteria, and . Using microsensor measurements for mapping the chemical environment, we show how promoted an alkalization of its local environment through degradation of amino acids and release of ammonia. When the two species were grown in proximity, the modified local environment induced a morphological change and growth of followed by sporulation. 2D spatial metabolomics enabled visualization and mapping of the degradation of oligopeptide structures by and morphological changes of through e.g. the release of membrane-associated metabolites. Proteome analysis and microscopy were used to validate the shift from vegetative growth towards sporulation. In summary, we demonstrate how environmental profiling by combined application of microsensor, microscopy, metabolomics and proteomics approaches can reveal growth and sporulation promoting effects resulting from interspecies interactions.
