Electronic transitions and ESIPT kinetics of the thienyl-3-hydroxychromone nucleobase surrogate in DNA duplexes: a DFT/MD-TDDFT study.
Alain Sougnabé, Daniel Lissouck, Fabien Fontaine-Vive, Mama Nsangou, Yves Mély, Alain Burger, Cyril A Kenfack
Author Information
Alain Sougnabé: Laboratoire d'Optique et Applications, Centre de Physique Atomique Moléculaire et Optique Quantique, Faculté des Sciences Université de Douala B. P. 8580 Douala Cameroon ckenf@yahoo.com.
Daniel Lissouck: Laboratoire d'Optique et Applications, Centre de Physique Atomique Moléculaire et Optique Quantique, Faculté des Sciences Université de Douala B. P. 8580 Douala Cameroon ckenf@yahoo.com.
Fabien Fontaine-Vive: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS, Parc Valrose 06108 Nice Cedex 2 France.
Mama Nsangou: Département de Physique, Faculté des Sciences Physiques, Ecole Normale Supérieure de Maroua, Université de Maroua Cameroon.
Yves Mély: Laboratoire de Bioimagerie et Pathologies, UMR 7021 du CNRS, Faculté de Pharmacie Faculté de Pharmacie, Université de Strasbourg 74, Route du Rhin 67401 Illkirch Cedex France. ORCID
Alain Burger: Institut de Chimie de Nice, UMR 7272, Université Côte d'Azur, CNRS, Parc Valrose 06108 Nice Cedex 2 France. ORCID
Cyril A Kenfack: Laboratoire d'Optique et Applications, Centre de Physique Atomique Moléculaire et Optique Quantique, Faculté des Sciences Université de Douala B. P. 8580 Douala Cameroon ckenf@yahoo.com. ORCID
The fluorescent nucleobase surrogate M (2-thienyl-3-hydroxychromone fluorophore) when imbedded in DNA opposite an abasic site exhibits a two colour response highly sensitive to environment changes and base composition. Its two colour emission originates from an excited state intramolecular proton transfer (ESIPT), which converts the excited normal N* form into its T* tautomer. To get deeper insight on the spectroscopic properties of M in DNA duplexes, quantum chemical calculations were performed on M stacked with different base pairs in model trimers extracted from MD simulations. The photophysics of M in duplexes appeared to be governed by stacking interactions as well as charge and hole transfer. Indeed, stacking of M in DNA screens M from H-bonding with water molecules, which favours ESIPT and thus, the emission of the T* form. With A and T flanking bases, the electronic densities in the frontier MOs were localized on M, in line with its effective absorption and emission. In addition, reduction of the free rotation between the thienyl and chromone groups together with the shielding of the dye from water molecules largely explain its enhanced quantum yield in comparison to the free M in solution. By contrast, the localisation of the electron density on the flanking G residues in the ground state and the energetically favorable hole transfer from M to G in the excited state explains the reduced quantum yield of M sandwiched between CG pairs. Finally, the much higher brightness of M as compared to 2-aminopurine when flanked by A and T residues could be related to the much stronger oscillator strength of its S → S transition and the ineffective charge transfer from M to A or T residues.
References
J Am Chem Soc. 2017 Feb 15;139(6):2520-2528
[PMID: 28112929]
