Culturing and patch clamping of Jurkat T cells and neurons on AlO coated nanowire arrays of altered morphology.
Jann Harberts, Robert Zierold, Cornelius Fendler, Aune Koitmäe, Parisa Bayat, Irene Fernandez-Cuesta, Gabriele Loers, Björn-Philipp Diercks, Ralf Fliegert, Andreas H Guse, Carsten Ronning, Gaute Otnes, Magnus Borgström, Robert H Blick
Author Information
Jann Harberts: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Robert Zierold: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Cornelius Fendler: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Aune Koitmäe: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Parisa Bayat: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Irene Fernandez-Cuesta: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975.
Gabriele Loers: Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf Falkenried 94 20251 Hamburg Germany.
Björn-Philipp Diercks: Department of Biochemistry and Molecular Cell Biology, The Calcium Signaling Group, University Medical Center Hamburg-Eppendorf Martinistraße 52 20251 Hamburg Germany.
Ralf Fliegert: Department of Biochemistry and Molecular Cell Biology, The Calcium Signaling Group, University Medical Center Hamburg-Eppendorf Martinistraße 52 20251 Hamburg Germany.
Andreas H Guse: Department of Biochemistry and Molecular Cell Biology, The Calcium Signaling Group, University Medical Center Hamburg-Eppendorf Martinistraße 52 20251 Hamburg Germany.
Carsten Ronning: Institute for Solid State Physics, Friedrich-Schiller-University Jena Helmholtzweg 3-5 07743 Jena Germany.
Gaute Otnes: NanoLund, Lund University Box 118 22100 Lund Sweden.
Magnus Borgström: NanoLund, Lund University Box 118 22100 Lund Sweden.
Robert H Blick: Center for Hybrid Nanostructures, Universität Hamburg Luruper Chaussee 149 22761 Hamburg Germany rblick@chyn.uni-hamburg.de +49 40 42838 1975. ORCID
Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on AlO coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.
