Enzyme-Linked Immunosorbent Assay (ELISA).

Mahdis Sadat Tabatabaei, Marya Ahmed
Author Information
  1. Mahdis Sadat Tabatabaei: Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada.
  2. Marya Ahmed: Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada. marahmed@upei.ca.

Abstract

Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides an overall summary of the basic principle of ELISA, discusses different components of ELISA assay, and clearly outline protocols for different types of ELISA assays, including direct, indirect, sandwich, competitive, and nanoparticle-based ELISA.

Keywords

References

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MeSH Term

Biotin
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity

Chemicals

Biotin

Word Cloud

Created with Highcharts 10.0.0ELISAassaydifferentassaystypesprotocolsAssayEnzyme-linkedimmunosorbentonespecificstraightforwarddetectingbiomoleculesresearchclinicsadvancesanalyticalmethodsconstantlyoptimizedimprovesensitivitynowavailabledetectvariousbiomarkerschapterprovidesoverallsummarybasicprinciplediscussescomponentsclearlyoutlineincludingdirectindirectsandwichcompetitivenanoparticle-basedEnzyme-LinkedImmunosorbentBiotin–streptavidininteractionsCompetitiveDirectIndirectNanoparticle-basedSandwich

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