Wei Liu, Jiawen Wang, Shan Li, Huaqian Zhang, Li Meng, Liping Liu, Wenxiang Ping, Chunmei Du
Rice blast caused by is one of the most destructive plant diseases. The secondary metabolites of have potential as biological control agents against . However, no commercial secondary antimicrobial products of have been found by gene prediction, and, particularly relevant for this study, a biocontrol agent obtained from has yet to be found. In this research, genomic analysis was used to predict the secondary metabolites of , and the ability to develop biocontrol pharmaceuticals rapidly was demonstrated. The complete genome of the HD-087 strain was sequenced and revealed a number of key functional gene clusters that contribute to the biosynthesis of active secondary metabolites. The crude extract of lipopeptides (CEL) predicted by NRPS gene clusters was extracted from the fermentation liquid of HD-087 by acid precipitation followed by methanol extraction, and surfactins, iturins, and fengycins were identified by liquid chromatography-mass spectrometry (LC-MS). , the CEL of this strain inhibited spore germination and appressorial formation of by destroying membrane integrity and through the leakage of cellular components. , this CEL reduced the disease index of rice blast by approximately 76.9% on detached leaves, whereas its control effect on leaf blast during pot experiments was approximately 60%. Thus, the CEL appears to be a highly suitable alternative to synthetic chemical fungicides for controlling .
