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Methods in molecular biology (Clifton, N.J.)
2022;2513 221-242.

A Universal Gene Expression System for Novel Yeast Species.

Dominik Mojzita, Anssi Rantasalo, Marja Ilmén
Author Information
  1. Dominik Mojzita: VTT Technical Research Centre of Finland Ltd, Espoo, Finland. dominik.mojzita@vtt.fi.
  2. Anssi Rantasalo: EniferBio Oy, Espoo, Finland.
  3. Marja Ilmén: VTT Technical Research Centre of Finland Ltd, Espoo, Finland.
PMID: 35781208 DOI: 10.1007/978-1-0716-2399-2_13

Abstract

The current progress in sequencing of genomes and characterization of new species provides an increasing list of yeasts that show interesting physiological properties; however, the lack of expression tools for these new hosts is prohibiting their broader use in research or industry. Recently, we developed a universal expression system (SES) functional in broad spectrum of fungal species, which represent a solution for feasible gene expression control and genetic manipulation in these novel hosts. Here, we describe three example approaches for DNA transformation and high-level heterologous gene expression, using the SES system, in three yeast species, where minimal knowledge or prior experience in genetic modifications is available.

Keywords

Genetic tools Heterologous gene expression Metabolic engineering Novel yeast species Protein production Synthetic expression system (SES )

References

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MeSH Term

Gene Editing
Gene Expression
Industry
Phylogeny
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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