Ruiping Xu, Shasha Zhou, Jiaxin Song, Haiying Zhong, Tianwen Zhu, Yuhua Gong, Yan Zhou, Yinbing Bian
As the widest cultivated edible mushroom worldwide, suffers serious yield and quality losses from heat stress during growth and development, and in our previous study, exogenous 2,4-Dichlorophenoxyacetic acid (2,4-D) was found to improve the thermotolerance of strain YS3357, but the molecular mechanism remains unclear. Here, we explored the potential protective mechanism of exogenous 2,4-D against heat stress by transcriptome analysis. 2,4-D possible improve the thermotolerance of through regulating antioxidant genes, transcription factors, energy-provision system, membrane fluidity, and cell wall remodeling. Furthermore, 2,4-D was also found to regulate the saturation levels of fatty acids and ATP content in mycelium under heat stress. This study proposed a regulatory network of 2,4-D in regulating response to heat stress, providing a theoretical basis for improving thermotolerance, and facilitating the understanding of the molecular mechanism of exogenous hormones in alleviating abiotic stress damage to macrofungi.
