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07 15, 2022;12 (1): 12069.

A van der Waals force-based adhesion study of stem cells exposed to cold atmospheric plasma jets.

Kobra Hajizadeh, Hassan Mehdian, Kamal Hajisharifi, Eric Robert
Author Information
  1. Kobra Hajizadeh: Physics department, Faculty of Science, South Tehran Branch, Islamic Azad University, Tehran, Iran.
  2. Hassan Mehdian: Department of Physics and Institute for Plasma Research, Kharazmi University, Tehran, 15614, Iran.
  3. Kamal Hajisharifi: Department of Physics and Institute for Plasma Research, Kharazmi University, Tehran, 15614, Iran. hajisharifi@khu.ac.ir.
  4. Eric Robert: UMR 7344, CNRS/Université d'Orléans, GREMI, Orléans, France.
PMID: 35840616 DOI: 10.1038/s41598-022-16277-1

Abstract

Cold atmospheric plasma has established its effect on cell adhesion. Given the importance of cell adhesion in stem cells, the current study investigates the effect of plasma treatment on Human Bone Marrow Mesenchymal Stem Cells (HBMMSCs) adhesion by which the differentiation and fate of cells are determined. In this paper, adhesion modification is considered not only for cell- ECM (Extra cellular Matrix), but also between suspended cells, and enhanced adhesions were found in both circumstances. Regarding the previous works, the increase of the cell-ECM adhesion during the plasma therapy was mostly attributed to the enhancement of the production and activity of integrin proteins. Nevertheless, considering the importance of van der Waals forces at the cellular level, the effect of cold plasma on VDWFs and so its effect on adhesion is investigated in this work for the first time, to the best of our knowledge. For this purpose, employing the semi-empirical methods, the role of the plasma therapy on the VDWF between the cells has been studied at three levels; (a) plasma-induced dipole formation, (b) Hammaker coefficient modification of culture medium, and c) cell roughness modification. For suspended cell condition, we conclude and support that van der Waals forces (VDWFs) enhancement has a key role in cell adhesion processes. We believe that, the present work gives a new physical insight in studying the plasma therapy method at the cellular level.

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MeSH Term

Cell Adhesion
Cell Differentiation
Humans
Integrins
Mesenchymal Stem Cells
Plasma Gases

Chemicals

Integrins
Plasma Gases
Journal Article
Article has an altmetric score of 37

Word Cloud

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