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Journal of fungi (Basel, Switzerland)
Jul 26, 2022;8 (8):

Adenylsuccinate Synthetase MoADE12 Plays Important Roles in the Development and Pathogenicity of the Rice Blast Fungus.

Zhen Zhang, Zhongna Hao, Rongyao Chai, Haiping Qiu, Yanli Wang, Jiaoyu Wang, Guochang Sun
Author Information
  1. Zhen Zhang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  2. Zhongna Hao: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  3. Rongyao Chai: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  4. Haiping Qiu: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  5. Yanli Wang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  6. Jiaoyu Wang: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
  7. Guochang Sun: State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
PMID: 35893147 DOI: 10.3390/jof8080780

Abstract

Purines are basic components of nucleotides in living organisms. In this study, we identified the ortholog of adenylosuccinate synthase in by screening for growth-defective T-DNA insertional mutants. Gene replacement was performed to investigate the biological role of . mutants were adenine auxotrophs that failed to produce conidia, and showed reduced perithecia formation and pathogenicity. Moreover, the mutant was hypersensitive to Congo red and oxidants, indicating that was required for cell wall integrity and oxidative stress resistance. Transcriptomic analysis identified the underlying mechanisms and indicated that several pathogenicity-related genes were regulated in the mutant. Therefore, our data suggest that the adenylosuccinate synthase is involved in the de novo AMP biosynthesis pathway and is important for conidiation and pathogenicity in the rice blast fungus.

Keywords

Magnaporthe oryzae adenylsuccinate synthetase asexual development pathogenicity

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Grants

  1. LGN19C140011/Agricultural public welfare project of Zhejiang Province
  2. 31900126/National Natural Science Foundation of China
  3. 2021C02010/Zhejiang Key Research and Development Program
  4. LZ20C140001/Zhejiang Provincial Natural Science Foundation
  5. 2021DG700024-KF202110/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products
Journal Article
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