Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels.
Happy Kurnia Permatasari, Fahrul Nurkolis, Hardinsyah Hardinsyah, Nurpudji Astuti Taslim, Nindy Sabrina, Faisal Maulana Ibrahim, Jodi Visnu, Dian Aruni Kumalawati, Sri Awalia Febriana, Toto Sudargo, Melvin Junior Tanner, Isma Kurniatanty, Vincentius Mario Yusuf, Ronald Rompies, Muhammad Rahimi Bahar, Holipah Holipah, Nelly Mayulu
Author Information
Happy Kurnia Permatasari: Department of Biochemistry and Biomolecular, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Fahrul Nurkolis: Department of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, Indonesia.
Hardinsyah Hardinsyah: Department of Applied Nutrition, IPB University, Bogor, Indonesia.
Nurpudji Astuti Taslim: Department of Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Nindy Sabrina: Department of Nutrition, Dietetics and Food, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, VIC, Australia.
Faisal Maulana Ibrahim: Pharmaceutical Analysis and Medicinal Chemistry, Universitas Padjadjaran, Sumedang, Indonesia.
Jodi Visnu: Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Dian Aruni Kumalawati: Department of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, Indonesia.
Sri Awalia Febriana: Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Toto Sudargo: Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Melvin Junior Tanner: Department of Nutrition, Faculty of Public Health, University of Indonesia, Depok, Indonesia.
Isma Kurniatanty: Department of Biological Sciences, Sunan Kalijaga State Islamic University, Yogyakarta, Indonesia.
Vincentius Mario Yusuf: Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
Ronald Rompies: Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.
Muhammad Rahimi Bahar: Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.
Holipah Holipah: Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.
Nelly Mayulu: Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.
Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of done in a previous preclinical trial. Six major compounds from sea grape () extract were evaluated using an approach against human pancreatic , , and to predict prospective anti-obesity candidates. The inhibitory activity of the extract reached 90.30 ± 0.40%, 1.75% lower than orlistat. The inhibitory assay of the extract was 84.07 ± 5.28%, while the inhibitory activity against was 81.67 ± 1.54%; both were lower than acarbose. We observe the effect of extract as anti-obesity with anti-aging by evaluating the obesity parameters in the human body for a 4-week period. There was a significant decrease in blood glucose, total cholesterol, low-density lipoprotein (LDL), triglycerides (TG), waist circumference, waist-hip ratio, and body weight ( < 0.05); PGC-1α and high-density lipoprotein (HDL) increased significantly ( = 0.000), in Group B when compared with Group A. Our study revealed that sea grape extract is a potent anti-obesity with an anti-aging reagent that does not produce any significant adverse effects.
