Regenerative Medicine to Improve Outcomes of Ventral Hernia Reconstruction (REPAIR Study) Phase 1: Find the Best Decellularization Protocol for the Human Dermis.
Michele Altomare, Luca Ferrario, Laura Benuzzi, Marta Cecilia Tosca, Marta Gipponi, Imam Khodri, Giovanni Sesana, Stefania Cimbanassi, Stefano Piero Bernardo Cioffi, Andrea Spota, Roberto Bini, Osvaldo Chiara, Andrea Mingoli
Author Information
Michele Altomare: Department of Surgical Sciences, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy. ORCID
Luca Ferrario: General Surgery Residency Program, State University of Milan, Via Festa del Perdono 7, 20122 Milan, Italy. ORCID
Laura Benuzzi: General Surgery Residency Program, State University of Milan, Via Festa del Perdono 7, 20122 Milan, Italy.
Marta Cecilia Tosca: Tissue Bank and Tissue Therapy Unit, Emergency and Acceptance Department, ASST Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Marta Gipponi: Tissue Bank and Tissue Therapy Unit, Emergency and Acceptance Department, ASST Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Imam Khodri: Tissue Bank and Tissue Therapy Unit, Emergency and Acceptance Department, ASST Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Giovanni Sesana: Tissue Bank and Tissue Therapy Unit, Emergency and Acceptance Department, ASST Niguarda Hospital, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Stefania Cimbanassi: General Surgery and Trauma Team, ASST GOM Niguarda, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Stefano Piero Bernardo Cioffi: General Surgery and Trauma Team, ASST GOM Niguarda, Piazza Ospedale Maggiore 3, 20162 Milan, Italy. ORCID
Andrea Spota: General Surgery and Trauma Team, ASST GOM Niguarda, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Roberto Bini: General Surgery and Trauma Team, ASST GOM Niguarda, Piazza Ospedale Maggiore 3, 20162 Milan, Italy. ORCID
Osvaldo Chiara: General Surgery and Trauma Team, ASST GOM Niguarda, Piazza Ospedale Maggiore 3, 20162 Milan, Italy.
Andrea Mingoli: Department of Surgery "P. Valdoni", Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy. ORCID
Background: Tissue regeneration is a complex process that allows wounds to heal. Many options are currently available to help human skin repair and to reduce the recurrence of hernias. The aim of this study is to analyze the best decellularization protocol for allogenic human dermal tissues. Methods: Dermal flaps from donors were used and compared with a control group. Each flap was subjected to seven different decellularization protocols and washed with a sequence of five solutions. The samples were then subjected to four control tests (such as Nile Red), and long-term contacts were analyzed to assess whether the decellularized dermis samples could support the growth of human fibroblasts. Results: All the samples had an average residual viability of 60%. Except for one sample, the decellularization treatments were able to reduce cell viability significantly. The Nile Red test showed a significant reduction in phospholipid content (mean 90%, p-value < 0.05) in all treatments. The cell growth increased in a linear manner. As described in the literature, sodium-dodecyl-sulfate (SDS) caused an interference between the test and the detergent. Conclusions: This paper shows the first step to finding the best decellularization protocol for allografting human dermal tissues. Further biocompatibility tests and DNA quantification are necessary.
