OpenLB
Open Library of Bioscience
Advanced Search
Polish journal of microbiology
Sep 01, 2022;71 (3): 421-428.

Whole Genome Sequence Analysis of Bacteriophage P2.

Hanfang Zhu, She Guo, Jie Zhao, Hafiz Arbab Sakandar, Ruirui Lv, Qiannan Wen, Xia Chen
Author Information
  1. Hanfang Zhu: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  2. She Guo: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  3. Jie Zhao: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  4. Hafiz Arbab Sakandar: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  5. Ruirui Lv: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  6. Qiannan Wen: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
  7. Xia Chen: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, P.R.China.
PMID: 36185020 DOI: 10.33073/pjm-2022-037

Abstract

Phage P2 was isolated from failed fermentation broth carried out by IMAU10120. A previous study in our laboratory showed that this phage belonged to the family. In this study, this phage's genomic characteristics were analyzed using whole-genome sequencing. It was revealed that phage P2 was 77.9 kb in length and had 39.28% G + C content. Its genome included 96 coding sequences (CDS) and two tRNA genes involved in the function of the structure, DNA replication, packaging, and regulation. Phage P2 had higher host specificity; many tested strains were not infected. Cell wall adsorption experiments showed that the adsorption receptor component of phage P2 might be a part of the cell wall peptidoglycan. This research might enrich the knowledge about genomic information of lactobacillus phages and provide some primary data to establish phage control measures.

Keywords

Lactiplantibacillus plantarum phage P2 adsorption receptor functional genes genome characteristics host specificity

References

  1. Nat Biotechnol. 2020 Nov;38(11):1309-1316 [PMID: 32665660]
  2. Nature. 2006 Feb 2;439(7076):621-4 [PMID: 16452983]
  3. Gigascience. 2012 Dec 27;1(1):18 [PMID: 23587118]
  4. Food Microbiol. 2013 May;34(1):81-7 [PMID: 23498181]
  5. Nat Biotechnol. 2019 May;37(5):540-546 [PMID: 30936562]
  6. Virus Res. 2020 Jul 2;283:197983 [PMID: 32325115]
  7. J Biotechnol. 2005 Jan 12;115(1):101-7 [PMID: 15607229]
  8. J Basic Microbiol. 2019 Feb;59(2):123-133 [PMID: 30485461]
  9. Front Microbiol. 2021 Jun 14;12:678012 [PMID: 34194413]
  10. J Appl Microbiol. 2004;96(2):340-51 [PMID: 14723695]
  11. Nature. 1983 Sep 29-Oct 5;305(5933):448-51 [PMID: 6312322]
  12. J Dairy Sci. 2019 May;102(5):3879-3886 [PMID: 30852005]
  13. Cell Host Microbe. 2019 Feb 13;25(2):219-232 [PMID: 30763536]
  14. Virus Evol. 2022 May 28;8(1):veac031 [PMID: 35646390]
  15. Cell. 2018 Mar 8;172(6):1260-1270 [PMID: 29522746]
  16. Protein Sci. 2012 Jun;21(6):809-18 [PMID: 22434504]
  17. World J Clin Cases. 2022 Jun 16;10(17):5518-5530 [PMID: 35979101]
  18. J Dairy Sci. 2006 Jul;89(7):2414-23 [PMID: 16772557]
  19. BMC Genomics. 2014 Oct 04;15:855 [PMID: 25280881]
  20. Appl Environ Microbiol. 2012 Dec;78(24):8719-34 [PMID: 23042172]
  21. J Appl Microbiol. 2000 Dec;89(6):1059-65 [PMID: 11123479]
  22. Microbiome Res Rep. 2022 Jan 12;1(1):3 [PMID: 38089066]
  23. Virus Res. 2019 Nov;273:197764 [PMID: 31550486]
  24. J Mol Biol. 2002 May 17;318(5):1395-404 [PMID: 12083526]
  25. Nat Rev Microbiol. 2015 Dec;13(12):777-86 [PMID: 26548913]
  26. Front Microbiol. 2022 Apr 25;13:853593 [PMID: 35547140]
  27. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4160-5 [PMID: 19251647]

MeSH Term

Bacteriophage P2
Bacteriophages
Genome, Viral
Peptidoglycan
Siphoviridae
Whole Genome Sequencing

Chemicals

Peptidoglycan
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0P2phageadsorptionPhagestudyshowedgenomiccharacteristicsgenomegeneshostspecificitywallreceptormightisolatedfailedfermentationbrothcarriedIMAU10120previouslaboratorybelongedfamilyphage'sanalyzedusingwhole-genomesequencingrevealed779kblength3928%G+Ccontentincluded96codingsequencesCDStwotRNAinvolvedfunctionstructureDNAreplicationpackagingregulationhighermanytestedstrainsinfectedCellexperimentscomponentpartcellpeptidoglycanresearchenrichknowledgeinformationlactobacillusphagesprovideprimarydataestablishcontrolmeasuresWholeGenomeSequenceAnalysisBacteriophageLactiplantibacillusplantarumfunctional

Similar Articles

Cited By