Metagenomic analysis of viromes in tissues of wild Qinghai vole from the eastern Tibetan Plateau.
Xiaozhou He, Xu Wang, Guohao Fan, Fan Li, Weiping Wu, Zhenghuan Wang, Meihua Fu, Xu Wei, Shuo Ma, Xuejun Ma
Author Information
Xiaozhou He: NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. hexz@ivdc.chinacdc.cn.
Xu Wang: National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai, People's Republic of China.
Guohao Fan: NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Fan Li: NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
Weiping Wu: National Institute of Parasitic Diseases, Chinese Center for Diseases Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai, People's Republic of China.
Zhenghuan Wang: School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.
Meihua Fu: Shanghai Municipal Center for Disease Control and Prevention, Shanghai, People's Republic of China.
Xu Wei: School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.
Shuo Ma: School of Life Sciences, East China Normal University, Shanghai, People's Republic of China.
Xuejun Ma: NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China. maxj@ivdc.chinacdc.cn.
Rodents are natural reservoirs of diverse zoonotic viruses and widely distributed on the Tibetan Plateau. A comprehensive understanding of the virome in local rodent species could provide baseline of viral content and assist in efforts to reduce the risk for future emergence of rodent related zoonotic diseases. A total of 205 tissue and fecal samples from 41 wild Qinghai voles were collected. Metagenomic analyses were performed to outline the characteristics of the viromes, and phylogenetic analyses were used to identify the novel viral genomes. The virome distribution among five tissues (liver, lung, spleen, small intestine with content and feces) was also compared. We identified sequences related to 46 viral families. Novel viral genomes from distinct evolutionary lineages with known viruses were characterized for their genomic and evolutionary characteristics, including Hepatovirus, Hepacivirus, Rotavirus, and Picobirnavirus. Further analyses revealed that the core virome harbored by rodent internal tissues were quite different from the virome found in intestine and fecal samples. These findings provide an overview of the viromes in wild Qinghai voles, which are unique and the most common rodent species in the eastern Tibetan Plateau. A high diversity of viruses is likely present in rodent species in this area.
References
Virus Evol. 2018 Mar 30;4(1):vey008
[PMID: 29644096]
