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Archives of microbiology
Oct 31, 2022;204 (11): 680.

Characterization of novel of temperate phages of Staphylococcus aureus isolated from bovine milk.

Bibiana Martins Barasuol, Juliana Felipetto Cargnelutti, Luis Antônio Sangioni, Daniela Isabel Brayer Pereira, Ana Paula Muterle Varela, Fabiana Quoos Mayer, Emanuele Serro Pottker, Gabriela Flores Gonçalves, Samuel Cibulski, Sônia de Avila Botton
Author Information
  1. Bibiana Martins Barasuol: Programa de Pós-Graduação em Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rural, Universidade Federal de Santa Maria, Avenida Roraima, Santa Maria, RS, 1000, Brazil.
  2. Juliana Felipetto Cargnelutti: Programa de Pós-Graduação em Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rural, Universidade Federal de Santa Maria, Avenida Roraima, Santa Maria, RS, 1000, Brazil.
  3. Luis Antônio Sangioni: Programa de Pós-Graduação em Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rural, Universidade Federal de Santa Maria, Avenida Roraima, Santa Maria, RS, 1000, Brazil.
  4. Daniela Isabel Brayer Pereira: Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
  5. Ana Paula Muterle Varela: Secretaria de Agricultura, Pecuária e Desenvolvimento Rural, Departamento de Diagnóstico e Pesquisa Agropecuária, Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Eldorado do Sul, RS, Brazil.
  6. Fabiana Quoos Mayer: Secretaria de Agricultura, Pecuária e Desenvolvimento Rural, Departamento de Diagnóstico e Pesquisa Agropecuária, Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Eldorado do Sul, RS, Brazil.
  7. Emanuele Serro Pottker: Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  8. Gabriela Flores Gonçalves: Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  9. Samuel Cibulski: Laboratório de Biotecnologia Celular e Molecular, Centro de Biotecnologia (Cbiotec), Universidade Federal da Paraíba, João Pessoa, PB, Brazil.
  10. Sônia de Avila Botton: Programa de Pós-Graduação em Medicina Veterinária, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rural, Universidade Federal de Santa Maria, Avenida Roraima, Santa Maria, RS, 1000, Brazil. sabott20@gmail.com. ORCID
PMID: 36315293 DOI: 10.1007/s00203-022-03296-9

Abstract

Bovine mastitis is an important disease in dairy cows, and Staphylococcus aureus is the most prevalent microorganism. Bacteriophages are considered an alternative to treat bacterial infections due to antimicrobial resistance crisis. In this study, we isolated and characterized novel S. aureus temperate phages, namely B_UFSM4 and B_UFSM5, from bovine milk. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, respectively. The viruses have double-stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus phage phiPV83, CN125 and JS01. Therefore, the phages were classified into the family Siphoviridae, genus Biseptimavirus and order Caudovirales. In the host range, the B_UFSM4 and B_UFSM5 had lytic activity of 45.8% and 54.16%, respectively, inclusive on isolates from Staphylococcus sciuri and Rothia terrae. Thus, in this study, species novel of S. aureus temperate phages was isolated and characterized, these phages reveal similarities to each other; however, they are distinct from other species of S. aureus phages of the family Siphoviridae.

Keywords

Bacteriophages Bovine mastitis Phages S. aureus

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Grants

  1. 001/Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

MeSH Term

Animals
Female
Cattle
Staphylococcus aureus
Milk
Staphylococcal Infections
Staphylococcus Phages
Mastitis, Bovine
Siphoviridae
Journal Article
Article has an altmetric score of 2

Word Cloud

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