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International microbiology : the official journal of the Spanish Society for Microbiology
May, 2023;26 (2): 231-242.

Bacterial communities associated with mushrooms in the Qinghai-Tibet Plateau are shaped by soil parameters.

Rui Xing, Hai-Chen Zhang, Qing-Bo Gao, Fa-Qi Zhang, Xiao-Feng Chi, Shi-Long Chen
Author Information
  1. Rui Xing: Key Laboratory of Adaptation, and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining, Qinghai, 810001, People's Republic of China. ORCID
  2. Hai-Chen Zhang: State Key Laboratory of Plateau Ecology, and Agriculture, Qinghai University, Xining, China.
  3. Qing-Bo Gao: Key Laboratory of Adaptation, and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining, Qinghai, 810001, People's Republic of China.
  4. Fa-Qi Zhang: Key Laboratory of Adaptation, and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining, Qinghai, 810001, People's Republic of China.
  5. Xiao-Feng Chi: Key Laboratory of Adaptation, and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining, Qinghai, 810001, People's Republic of China.
  6. Shi-Long Chen: Key Laboratory of Adaptation, and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 59 Xiguan Avenue, Xining, Qinghai, 810001, People's Republic of China. slchen@nwipb.cas.cn.
PMID: 36352292 DOI: 10.1007/s10123-022-00286-1

Abstract

Fungi capable of producing fruit bodies are essential food and medicine resources. Despite recent advances in the study of microbial communities in mycorrhizospheres, little is known about the bacterial communities contained in fruit bodies. Using high-throughput sequencing, we investigated the bacterial communities in four species of mushrooms located on the alpine meadow and saline-alkali soil of the Qinghai-Tibet Plateau (QTP). Proteobacteria (51.7% on average) and Actinobacteria (28.2% on average) were the dominant phyla in all of the sampled fairy ring fruit bodies, and Acidobacteria (27.5% on average) and Proteobacteria (25.7% on average) dominated their adjacent soils. For the Agria. Bitorquis, Actinobacteria was the dominant phylum in its fruit body (67.5% on average) and adjacent soils (65.9% on average). The alpha diversity (i.e., Chao1, Shannon, Richness, and Simpson indexes) of the bacterial communities in the fruit bodies were significantly lower than those in the soil samples. All of the fungi shared more than half of their bacterial phyla and 16.2% of their total operational taxonomic units (OTUs) with their adjacent soil. Moreover, NH and pH were the key factors associated with bacterial communities in the fruit bodies and soils, respectively. These results indicate that the fungi tend to create a unique niche that selects for specific members of the bacterial community. Using culture-dependent methods, we also isolated 27 bacterial species belonging to three phyla and five classes from fruit bodies and soils. The strains isolated will be useful for future research on interactions between mushroom-forming fungi and their bacterial endosymbionts.

Keywords

Bacterial endosymbionts High throughput sequencing Microbial interactions Mycorrhiza helper bacteria Qinghai-Tibet Plateau

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Grants

  1. (LHZX-2020-02-01/The Sanjiangyuan National Park Joint Program
  2. QHTX- 2020- 004/Special fund for Qilian Mountain National Park
  3. XDA2005010406/Strategic Priority Research Program of the Chinese Academy of Sciences
  4. 2022-ZJ-Y04/Construction Project for Innovation Platform of Qinghai province

MeSH Term

Tibet
Soil
Agaricales
Bacteria
Microbiota
Soil Microbiology

Chemicals

Soil
Journal Article

Word Cloud

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