Multi-Organ Transcriptome Response of Lumpfish () to Subspecies Systemic Infection.
Setu Chakraborty, Ahmed Hossain, Trung Cao, Hajarooba Gnanagobal, Cristopher Segovia, Stephen Hill, Jennifer Monk, Jillian Porter, Danny Boyce, Jennifer R Hall, Gabriela Bindea, Surendra Kumar, Javier Santander
Author Information
Setu Chakraborty: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada. ORCID
Ahmed Hossain: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Trung Cao: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Hajarooba Gnanagobal: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada. ORCID
Cristopher Segovia: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Stephen Hill: Cold-Ocean Deep-Sea Research Facility, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Jennifer Monk: Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Jillian Porter: Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Danny Boyce: Dr. Joe Brown Aquatic Research Building, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Jennifer R Hall: Aquatic Research Cluster, CREAIT Network, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Gabriela Bindea: INSERM, Laboratory of Integrative Cancer Immunology, 75006 Paris, France.
Surendra Kumar: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Javier Santander: Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada. ORCID
Lumpfish is utilized as a cleaner fish to biocontrol sealice infestations in Atlantic salmon farms. , a Gram-negative facultative intracellular pathogen, is the causative agent of furunculosis in several fish species, including lumpfish. In this study, lumpfish were intraperitoneally injected with different doses of to calculate the LD. Samples of blood, head-kidney, spleen, and liver were collected at different time points to determine the infection kinetics. We determined that LD is 10 CFU per dose. We found that the lumpfish head-kidney is the primary target organ of . Triplicate biological samples were collected from head-kidney, spleen, and liver pre-infection and at 3- and 10-days post-infection for RNA-sequencing. The reference genome-guided transcriptome assembly resulted in 6246 differentially expressed genes. The assembly resulted in 403,204 transcripts, which added 1307 novel genes not identified by the reference genome-guided transcriptome. Differential gene expression and gene ontology enrichment analyses suggested that induces lethal infection in lumpfish by uncontrolled and detrimental blood coagulation, complement activation, inflammation, DNA damage, suppression of the adaptive immune system, and prevention of cytoskeleton formation.
