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Applied biochemistry and biotechnology
Apr, 2023;195 (4): 2414-2431.

Histopathological and Biochemical Changes in the Gills of Anabas testudineus on Exposure to Polycyclic Aromatic Hydrocarbon Naphthalene.

Susri Nayak, Lipika Patnaik
Author Information
  1. Susri Nayak: Environmental Science Laboratory, Department of Zoology, Centre of Excellence in Environment and Public Health, Ravenshaw University, Cuttack, 753008, Odisha, India.
  2. Lipika Patnaik: Environmental Science Laboratory, Department of Zoology, Centre of Excellence in Environment and Public Health, Ravenshaw University, Cuttack, 753008, Odisha, India. lipika_pat@yahoo.co.uk. ORCID
PMID: 36383310 DOI: 10.1007/s12010-022-04214-x

Abstract

Naphthalene, a polycyclic aromatic hydrocarbon, is generated by various distillation, petroleum, and coal-tar production units and is used worldwide as mothballs, soil fumigants, and toilet deodorants. Considering the susceptibility of aquatic animals to different types of stressors in several water bodies, this study was carried out to evaluate the impact of naphthalene on the architecture of gill tissue including response of various enzymes like cholinesterase (ChE) activity, lactate dehydrogenase (LDH) activity, and lipid peroxidation (LPX) level of the freshwater fish Anabas testudineus. Activities of antioxidants like catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH) were also evaluated. Constant loss of gill structure and secondary lamellar fusion was observed in fishes exposed to various concentrations of naphthalene. ChE, LDH, LPx, CAT, Gpx and GSH activities indicated significant variation (p < 0.05) between the control and experimental groups. ChE activity was lowered in experimental fishes; however, LDH activity, LPx levels, and CAT activity were elevated in response to various concentrations of naphthalene as compared to control group. Both GPx and GSH activities decreased in the gill tissue of the experimental fishes. Thus, a conclusion was drawn that naphthalene is a potent toxicant capable of inflicting tissue damage leading to physiological changes in the exposed fishes.

Keywords

Anabas testudineus Cholinesterase Gill tissues Lactate dehydrogenase Lipid peroxidation Naphthalene

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Grants

  1. F.No. 33/2012/University Grants Commission
  2. S-SCST-MISC-0054-2018-1152/DST, Odisha Biju Patnaik Fellowship
  3. HE-PTC-WB02017/OHEPEE

MeSH Term

Animals
Gills
Polycyclic Aromatic Hydrocarbons
Fishes
Antioxidants
Catalase
Glutathione
Lipid Peroxidation
Naphthalenes
Oxidative Stress
Glutathione Peroxidase

Chemicals

Polycyclic Aromatic Hydrocarbons
Antioxidants
Catalase
Glutathione
naphthalene
Naphthalenes
Glutathione Peroxidase
Journal Article

Word Cloud

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