A Complete Genome of NC_YFY_NT001 and Pan-Genomic Analysis Based on Different Sources of . Isolates Reveal Possibly Host-Related Virulence Factors.
Qinming Cai, Yongcheng Huang, Li Zhou, Niya Hu, Yanling Liu, Fujia Guo, Qiong Liu, Xiaotian Huang, Yunyi Zhang, Lingbing Zeng
Author Information
Qinming Cai: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China.
Yongcheng Huang: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China. ORCID
Li Zhou: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China.
Niya Hu: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China.
Yanling Liu: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China.
Fujia Guo: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China. ORCID
Qiong Liu: Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, People's Republic of China.
Xiaotian Huang: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China.
Yunyi Zhang: Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, People's Republic of China.
Lingbing Zeng: The First Affiliated Hospital of Nanchang University, School of Public Health, Nanchang University, Nanchang, People's Republic of China. ORCID
Objective: We aimed to identify the possible virulence genes associated with NC_YFY_NT001 isolated by ourselves and other . Methods: The genome of NC_YFY_NT001 was completed by using PacBio and Illumina platforms. A pan-genomic analysis was applied to selected complete genomes. Results: NC_YFY_NT001 can cause healthy mice death by tail intravenous injection. The genome of NT001 has one circular chromosome 8,850,000 bp and one circular plasmid 70,000 bp with ~68% GC content. The chromosome and plasmid encode 7914 and 80 proteins, respectively. Furthermore, a pan-genomic analysis showed a total of 45,825 gene clusters, then 304 core, 21,045 shell and 24,476 cloud gene clusters were classified using specific parameters. In addition, we found that catalases were more abundant in human isolates. Furthermore, we also found no significant differences in the MCE proteins between different strains from different sources. The pan-genomic analysis also showed that 67 genes could only be found in humoral isolates. ReX3 and DUF853 domain protein were found in all eight human isolates. The composition of unique genes in humoral isolate genomes indicated that the transcriptional regulators may be important when invades the host, which allows them to survive in the new ecological system. Conclusion: In this study, we confirmed that NT001 could cause infected animal death, and identified many possible virulence factors for our future studies. This study also provides new insight for our further study on virulence mechanisms.
