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Antibiotics (Basel, Switzerland)
Nov 23, 2022;11 (12):

Molecular Epidemiology of Antimicrobial Resistance and Virulence Profiles of , spp., and spp. Isolated from Coastal Seawater for Aquaculture.

Saharuetai Jeamsripong, Varangkana Thaotumpitak, Saran Anuntawirun, Nawaphorn Roongrojmongkhon, Edward R Atwill, Woranich Hinthong
Author Information
  1. Saharuetai Jeamsripong: Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand. ORCID
  2. Varangkana Thaotumpitak: Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  3. Saran Anuntawirun: Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  4. Nawaphorn Roongrojmongkhon: Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
  5. Edward R Atwill: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California-Davis, Davis, CA 95616, USA.
  6. Woranich Hinthong: Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok 10210, Thailand. ORCID
PMID: 36551345 DOI: 10.3390/antibiotics11121688

Abstract

The occurrence of waterborne antimicrobial-resistant (AMR) bacteria in areas of high-density oyster cultivation is an ongoing environmental and public health threat given the popularity of shellfish consumption, water-related human recreation throughout coastal Thailand, and the geographical expansion of Thailand's shellfish industry. This study characterized the association of phenotypic and genotypic AMR, including extended-spectrum β-lactamase (ESBL) production, and virulence genes isolated from waterborne () ( = 84), () subsp. ( = 12), () ( = 249), and () ( = 39) from Thailand's coastal aquaculture regions. All (100.0%) and half of (51.3%) isolates harbored their unique virulence gene, and , respectively. The majority of isolates of and , ~25% of subsp. , and ~12% of , exhibited phenotypic AMR to multiple antimicrobials, with 8.9% of all coastal water isolates exhibiting multidrug resistance (MDR). Taken together, we recommend that coastal water quality surveillance programs include monitoring for bacterial AMR for food safety and recreational water exposure to water for Thailand's coastal water resources.

Keywords

E. coli ESBL Salmonella V. cholerae V. parahaemolyticus antimicrobial resistance coastal aquaculture resistance genes

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Grants

  1. CU_FRB640001_01_31_9/Thailand Science Research and Innovation
Journal Article

Word Cloud

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