OpenLB
Open Library of Bioscience
Advanced Search
International journal of molecular sciences
Dec 16, 2022;23 (24):

Genome-Wide Analysis of Circular RNAs Reveals circCHRNG Regulates Sheep Myoblast Proliferation via miR-133/SRF and MEF2A Axis.

Yue Liu, Qian Chen, Jingjing Bao, Yabin Pu, Jianlin Han, Huijing Zhao, Yuehui Ma, Qianjun Zhao
Author Information
  1. Yue Liu: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  2. Qian Chen: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  3. Jingjing Bao: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  4. Yabin Pu: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  5. Jianlin Han: CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China.
  6. Huijing Zhao: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  7. Yuehui Ma: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  8. Qianjun Zhao: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
PMID: 36555706 DOI: 10.3390/ijms232416065

Abstract

As relatively new members of the non-coding RNA family, circRNAs play important roles in a variety of biological processes. However, the temporal expression pattern and the function of circRNAs during sheep skeletal muscle development remains unclear. This study aimed to identify circRNAs related to sheep skeletal muscle development and explore their roles in myoblast proliferation. The circRNA expression profiles of longissimus dorsi of sheep from F90, L30, and A3Y were obtained by the RNA-seq method. The function and mechanisms of the novel circCHRNG in muscle satellite cell proliferation were explored using CCK-8 assay, Western blot, qPCR, and dual-luciferase reporter assay. We identified 12,375 circRNAs, including 476, 133, and 233 DEcircRNAs found among three comparative groups. KEGG results showed that DEcircRNAs were enriched in muscle contraction, the regulation of cell proliferation, and the AMPK, insulin, and PI3K-Akt signaling pathways. Notably, a novel circRNA, termed circRNA CHRNG, acts as a miR-133 sponge to promote skeletal muscle satellite cell proliferation. Our study provides a systematic description of circRNAs of ovine skeletal muscle across fetal, lamb, and adult stages. GO and KEGG analyses showed that DEcircRNAs were enriched in multiple pathways associated with muscle development, such as the PI3K-Akt and AMPK signaling pathways. In addition, we propose that circCHRNG acts as a miR-133 sponge to upregulate the expression levels of SRF and MEF2A, thereby promoting myoblast proliferation.

Keywords

RNA-seq ceRNA circCHRNG circRNA skeletal muscle

References

  1. DNA Res. 2018 Feb 1;25(1):71-86 [PMID: 29036326]
  2. Sheng Wu Gong Cheng Xue Bao. 2022 Sep 25;38(9):3131-3140 [PMID: 36151789]
  3. Trends Neurosci. 2016 Sep;39(9):597-604 [PMID: 27445124]
  4. BMC Genet. 2016 May 31;17(1):71 [PMID: 27245440]
  5. FASEB J. 2015 Jul;29(7):3054-64 [PMID: 25868726]
  6. Nature. 2012 Feb 15;482(7385):339-46 [PMID: 22337053]
  7. Clin Hemorheol Microcirc. 2022;82(2):169-182 [PMID: 35662113]
  8. Nat Commun. 2021 Jun 17;12(1):3715 [PMID: 34140474]
  9. PLoS One. 2011;6(12):e29173 [PMID: 22195016]
  10. Nat Genet. 2006 Feb;38(2):228-33 [PMID: 16380711]
  11. Front Cell Dev Biol. 2021 Mar 05;9:619329 [PMID: 33748107]
  12. Cell J. 2020 Jul;22(Suppl 1):133-140 [PMID: 32779443]
  13. Biomed Res Int. 2015;2015:676575 [PMID: 26258142]
  14. Int J Cardiol. 2019 Oct 1;292:188-196 [PMID: 30967276]
  15. Int J Biochem Cell Biol. 2018 Jun;99:211-218 [PMID: 29684477]
  16. Mol Cell. 2017 Apr 6;66(1):22-37.e9 [PMID: 28344082]
  17. Nat Cell Biol. 2006 Mar;8(3):278-84 [PMID: 16489342]
  18. Exp Ther Med. 2021 Aug;22(2):791 [PMID: 34093747]
  19. Asian-Australas J Anim Sci. 2018 Jun;31(6):812-819 [PMID: 29268579]
  20. Biomed Res Int. 2015;2015:391306 [PMID: 26221592]
  21. Mol Cell Biol. 2004 Nov;24(21):9295-304 [PMID: 15485899]
  22. Noncoding RNA. 2021 Aug 04;7(3): [PMID: 34449657]
  23. Dev Biol. 2016 Feb 1;410(1):1-13 [PMID: 26708096]
  24. Biochem Biophys Res Commun. 2018 Sep 5;503(2):863-869 [PMID: 29928876]
  25. Int J Mol Sci. 2019 Mar 04;20(5): [PMID: 30836719]
  26. Cell Death Dis. 2013 Nov 28;4:e934 [PMID: 24287695]
  27. Cell Tissue Res. 2021 Feb;383(2):795-807 [PMID: 33151455]
  28. DNA Res. 2017 Oct 1;24(5):523-535 [PMID: 28575165]
  29. EMBO Rep. 2005 Jun;6(6):570-6 [PMID: 15905852]
  30. Development. 2013 Jan 1;140(1):31-42 [PMID: 23154418]
  31. Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20844-9 [PMID: 18093911]
  32. Genome Biol. 2015 Nov 05;16:245 [PMID: 26541409]
  33. Cell. 2011 Aug 5;146(3):353-8 [PMID: 21802130]
  34. Mol Cancer. 2017 May 23;16(1):94 [PMID: 28535767]
  35. Cell Mol Life Sci. 2014 Nov;71(22):4361-71 [PMID: 25080109]
  36. PLoS One. 2018 Apr 26;13(4):e0196255 [PMID: 29698438]
  37. Anim Biosci. 2022 Sep 07;: [PMID: 36108684]
  38. Front Cell Dev Biol. 2019 Nov 19;7:289 [PMID: 31803743]
  39. Wiley Interdiscip Rev RNA. 2022 Jan;13(1):e1685 [PMID: 34342387]
  40. Cell Death Dis. 2019 Feb 22;10(3):182 [PMID: 30796204]
  41. Nature. 2005 Jul 14;436(7048):214-20 [PMID: 15951802]
  42. J Cell Physiol. 2019 Apr;234(4):3490-3499 [PMID: 30471101]
  43. Cancer Lett. 2018 Apr 1;418:41-50 [PMID: 29330104]
  44. Nature. 2013 Mar 21;495(7441):384-8 [PMID: 23446346]
  45. J Agric Food Chem. 2022 Jul 6;70(26):8145-8163 [PMID: 35749701]
  46. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3852-6 [PMID: 1069269]
  47. Cell Death Dis. 2017 Oct 26;8(10):e3153 [PMID: 29072698]
  48. Mol Cell. 2013 Sep 26;51(6):792-806 [PMID: 24035497]

Grants

  1. No.32172701/National Natural Science Foundation of China
  2. 2021YFD1300901/National Key R&D Program of China
  3. CARS-39-01/the Modern Wool Sheep Industry System

MeSH Term

Animals
Sheep
RNA, Circular
MicroRNAs
Proto-Oncogene Proteins c-akt
Phosphatidylinositol 3-Kinases
AMP-Activated Protein Kinases
Cell Proliferation
Myoblasts

Chemicals

RNA, Circular
MicroRNAs
Proto-Oncogene Proteins c-akt
Phosphatidylinositol 3-Kinases
AMP-Activated Protein Kinases
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0musclecircRNAsskeletalproliferationcircRNAcircCHRNGexpressionsheepdevelopmentcellDEcircRNAspathwaysrolesfunctionstudymyoblastRNA-seqnovelsatelliteassayKEGGshowedenrichedAMPKPI3K-AktsignalingactsmiR-133spongeMEF2Arelativelynewmembersnon-codingRNAfamilyplayimportantvarietybiologicalprocessesHowevertemporalpatternremainsunclearaimedidentifyrelatedexploreprofileslongissimusdorsiF90L30A3YobtainedmethodmechanismsexploredusingCCK-8WesternblotqPCRdual-luciferasereporteridentified12375including476133233foundamongthreecomparativegroupsresultscontractionregulationinsulinNotablytermedCHRNGpromoteprovidessystematicdescriptionovineacrossfetallambadultstagesGOanalysesmultipleassociatedadditionproposeupregulatelevelsSRFtherebypromotingGenome-WideAnalysisCircularRNAsRevealsRegulatesSheepMyoblastProliferationviamiR-133/SRFAxisceRNA

Similar Articles

Cited By (2)