LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway.
Shuai Shao, Yu Zhang, Feng Zhou, Xiaoxiang Meng, Zhenjun Yu, Guantong Li, Lina Zheng, Kun Zhang, Yuhan Li, Beichen Guo, Qi Liu, Mengxia Zhang, Xiaoxiao Du, Wei Hong, Tao Han
Author Information
Shuai Shao: The School of Medicine, Nankai University, Tianjin 300071, China.
Yu Zhang: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Feng Zhou: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Xiaoxiang Meng: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Zhenjun Yu: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Guantong Li: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Lina Zheng: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Kun Zhang: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Yuhan Li: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Beichen Guo: Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Qi Liu: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Mengxia Zhang: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Xiaoxiao Du: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Wei Hong: Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China.
Tao Han: The School of Medicine, Nankai University, Tianjin 300071, China.
acute liver injury is a common and serious syndrome caused by multiple factors and unclear pathogenesis. If the injury persists, liver injury can lead to cirrhosis and liver failure and ultimately results in the development of liver cancer. Emerging evidence has indicated that long noncoding RNAs (lncRNAs) play an important role in the development of liver injury. However, the role of antisense Igf2r RNA (Airn) in acute liver injury and its underlying mechanism remain largely unclear. In this study, we show that Airn is upregulated in liver tissue and primary hepatocytes from an acute liver injurymouse model. Consistently, Airn is also overexpressed in serum samples of patients with acute-on-chronic liver failure and is negatively correlated with the Model for End-Stage Liver Disease (MELD) score. Moreover, gene knockout and rescue assays reveal that Airn alleviates CCl -induced liver injury by inhibiting hepatocyte apoptosis and oxidative stress . Further investigation reveals that Airn decreases H O -induced hepatocyte apoptosis . Mechanistically, we reveal that Airn represses CCl - and H O -induced enhancement of phosphorylation of p65 and IκBα, suggesting that Airn inhibits hepatocyte apoptosis by inactivating the NF-κB pathway. In conclusion, our results demonstrate that Airn can alleviate acute liver injury by inhibiting hepatocyte apoptosis via inactivating the NF-κB signaling pathway, and Airn could be a potential biomarker for acute liver injury.
