Apoptosis Effects of L. Extract on Human MCF-7 Breast Cancer Cell Line:.
Amir Reza Gholipour, Leila Jafari, Mahsa Ramezanpour, Mehdi Evazalipour, Maral Chavoshi, Fatemeh Yousefbeyk, Saghi Jani Kargar Moghaddam, Mohammad Hossein Yekta Kooshali, Nahid Ramezanpour, Puyan Daei, Saeed Ghasemi, Masoud Hamidi
Author Information
Amir Reza Gholipour: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Leila Jafari: Pediatric Cell and Gene Therapy Research Center, Gene, Cell and Tissue Research Institute, Tehran University of Medical Science Tehran, Iran.
Mahsa Ramezanpour: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Mehdi Evazalipour: Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Maral Chavoshi: Department of Genetics and Developmental Biology, University of Vienna, Vienna, Austria.
Fatemeh Yousefbeyk: Department of Pharmacognosy, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Saghi Jani Kargar Moghaddam: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Mohammad Hossein Yekta Kooshali: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Nahid Ramezanpour: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Puyan Daei: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Saeed Ghasemi: Department of Medicinal Chemistry, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Masoud Hamidi: Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
Recently, the non-toxic properties of natural plant products have gained more focus as anticancer agents. Therefore, this study aimed to assess the apoptosis effects of the ethanolic extract of on the MCF-7 breast cancer cell line. In this experimental study, aerial parts of were collected in Lahijan city (Iran), and after confirmation, they were dried and extracted with ethanol for 24 h. Then, the total phenolic and flavonoid contents of the extract were measured. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay was used to measure the antioxidant properties of the extract. Selected cell lines (MCF-7 and human dermal fibroblast) were cultured in 6-wells dishes (1×10 cells/well). After 72 h of treating the extract, cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The expression of apoptotic genes (such as , , , and ) was studied by real-time polymerase chain reaction (PCR). The extract's total phenolic content was 31.30±02 μg of gallic acid equivalents/mg of dry extract, and the total flavonoid content was 49.61±04 μg of quercetin as equivalents/mg of extract. The antioxidant activity of was measured at the dose of 619.2 μg/μl, indicating that it decreases cancer cell viability and enhances apoptosis. Within the half maximal inhibitory concentrations, real-time PCR revealed substantial increases in (P<0.001), (P<0.05), and expression (P<0.05) in MCF-7 cells treated with . This study suggests that may cause apoptosis by oxidative stress in cancer cells.[GMJ.2022;11:e2484].
