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Jan 30, 2023;14 (2):

Enhancement of Pathogen Toxicity by Feeding Snyder Sonicated Bacteria Expressing Double-Stranded RNA That Interferes with Olfaction.

Dabao Jiang, Xiaoyu Lu, Ling Zhang, Fang Tang
Author Information
  1. Dabao Jiang: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
  2. Xiaoyu Lu: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
  3. Ling Zhang: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
  4. Fang Tang: Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
PMID: 36835709 DOI: 10.3390/insects14020140

Abstract

Snyder is a serious pest in China, and the odorant receptor co-receptor gene plays a crucial role in olfaction. However, the function of in the resistance of termites to entomopathogens has not been reported. We constructed ds-HT115 engineered bacteria based on the sequence from the full-length transcriptome data of . The engineered bacteria expressed dsRNA of . Sonication was used to inactivate the dsRNA-HT115 strain and obtain a large amount of ds. The ds produced using this method overcame the problem that genetically engineered bacteria could not be applied directly and improved its effectiveness against termites. Bioassays using the ds generated using this method showed that ds significantly increased the toxicity of the bacterial and fungal pathogens to . The present study showed, for the first time, the function of in termite resistance to pathogens, and the results provide a theoretical basis for the development and application of termite RNA biopesticides.

Keywords

Orco RNA interference biocontrol pathogen termite

References

  1. BMC Genet. 2020 Dec 18;21(Suppl 2):140 [PMID: 33339492]
  2. Nat Ecol Evol. 2018 Mar;2(3):557-566 [PMID: 29403074]
  3. Pest Manag Sci. 2021 Apr;77(4):1674-1682 [PMID: 33205592]
  4. Bioinform Biol Insights. 2012;6:33-47 [PMID: 22493562]
  5. Annu Rev Entomol. 2020 Jan 7;65:293-311 [PMID: 31610134]
  6. PLoS One. 2013 Jul 22;8(7):e69070 [PMID: 23894408]
  7. Science. 2009 May 8;324(5928):758 [PMID: 19423819]
  8. Nat Commun. 2014 May 20;5:3636 [PMID: 24845553]
  9. Dev Comp Immunol. 2012 Feb;36(2):372-7 [PMID: 21824492]
  10. PLoS One. 2015 Jul 14;10(7):e0132631 [PMID: 26171783]
  11. Front Physiol. 2019 Jun 11;10:714 [PMID: 31244679]
  12. Insect Biochem Mol Biol. 2016 Feb;69:82-90 [PMID: 25747010]
  13. J Insect Physiol. 2014 Jun;65:51-6 [PMID: 24831178]
  14. J Virol Methods. 2009 Dec;162(1-2):213-7 [PMID: 19712700]
  15. Mol Psychiatry. 2001 Nov;6(6):665-70 [PMID: 11673794]
  16. Methods. 2001 Dec;25(4):386-401 [PMID: 11846608]
  17. J Insect Physiol. 2010 Mar;56(3):227-35 [PMID: 19837076]
  18. Insect Biochem Mol Biol. 2020 Dec;127:103497 [PMID: 33188923]
  19. Cell. 2017 Aug 10;170(4):736-747.e9 [PMID: 28802043]
  20. Pest Manag Sci. 2021 Jun;77(6):2645-2658 [PMID: 33440063]
  21. Nature. 2000 Nov 16;408(6810):325-30 [PMID: 11099033]
  22. BMC Genomics. 2018 Jul 24;19(1):550 [PMID: 30041601]
  23. Pest Manag Sci. 2022 Jul;78(7):3117-3127 [PMID: 35442542]
  24. Curr Biol. 2008 Aug 5;18(15):1153-5 [PMID: 18674910]
  25. Pestic Biochem Physiol. 2022 Jul;185:105120 [PMID: 35772836]
  26. Insect Mol Biol. 2006 Oct;15(5):645-56 [PMID: 17069638]
  27. Nature. 2018 Aug;560(7719):447-452 [PMID: 30111839]
  28. Pest Manag Sci. 2020 Mar;76(3):1020-1030 [PMID: 31503391]
  29. Sci Rep. 2016 Jul 12;6:29620 [PMID: 27403935]
  30. Nucleic Acids Res. 2003 Aug 15;31(16):e93 [PMID: 12907745]
  31. Naturwissenschaften. 1999 Nov;86(11):544-8 [PMID: 10551951]
  32. Curr Opin Insect Sci. 2018 Aug;28:19-25 [PMID: 30551763]
  33. Insect Biochem Mol Biol. 2008 Aug;38(8):805-15 [PMID: 18625404]
  34. J Invertebr Pathol. 2011 Sep;108(1):1-6 [PMID: 21683707]
  35. PLoS One. 2012;7(10):e47412 [PMID: 23077609]
  36. Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4499-504 [PMID: 16537425]
  37. J Invertebr Pathol. 2008 Feb;97(2):165-70 [PMID: 17949740]
  38. Annu Rev Entomol. 2013;58:373-91 [PMID: 23020622]

Grants

  1. 20KJA220003/The Natural Science Foundation of the Jiangsu Higher Education Institutions of China
  2. not applicable/the Priority Academic Program Development Fund of Jiangsu Higher Education Institutions
Journal Article
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