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Mar 17, 2023;26 (3): 106147.

Duox is the primary NADPH oxidase responsible for ROS production during adult caudal fin regeneration in zebrafish.

Kunal Chopra, Milda Folkmanaitė, Liam Stockdale, Vishali Shathish, Shoko Ishibashi, Rachel Bergin, Jorge Amich, Enrique Amaya
Author Information
  1. Kunal Chopra: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  2. Milda Folkmanaitė: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  3. Liam Stockdale: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  4. Vishali Shathish: Manchester Fungal Infection Group (MFIG), Division of Evolution, Infection, and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  5. Shoko Ishibashi: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  6. Rachel Bergin: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  7. Jorge Amich: Manchester Fungal Infection Group (MFIG), Division of Evolution, Infection, and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
  8. Enrique Amaya: Division of Cell Matrix Biology & Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
PMID: 36843843 DOI: 10.1016/j.isci.2023.106147

Abstract

Sustained elevated levels of reactive oxygen species (ROS) have been shown to be essential for regeneration in many organisms. This has been shown primarily via the use of pharmacological inhibitors targeting the family of NADPH oxidases (NOXes). To identify the specific NOXes involved in ROS production during adult caudal fin regeneration in zebrafish, we generated mutants for , and (a key subunit of NOXes 1-4) and crossed these lines with a transgenic line ubiquitously expressing , which permits the measurement of ROS levels. Homozygous mutants had the greatest effect on ROS levels and rate of fin regeneration among the single mutants. However, : double mutants showed a greater effect on fin regeneration than the single mutants, suggesting that Nox1-4 also play a role during regeneration. This work also serendipitously found that ROS levels in amputated adult zebrafish fins oscillate with a circadian rhythm.

Keywords

Cell biology Ichthyology Molecular genetics

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Grants

  1. MR/L007525/1/Medical Research Council
Journal Article

Word Cloud

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