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01 24, 2023;15 (2):

Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil.

Lia Cunha, Adriana Luchs, Lais S Azevedo, Vanessa C M Silva, Marcilio F Lemos, Antonio C Costa, Adriana P Compri, Yasmin França, Ellen Viana, Fernanda Malta, Roberta S Medeiros, Raquel Guiducci, Simone G Morillo, Michele S Gomes-Gouvea, Deyvid Amgarten, João R R Pinho, Regina C Moreira
Author Information
  1. Lia Cunha: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  2. Adriana Luchs: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil. ORCID
  3. Lais S Azevedo: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  4. Vanessa C M Silva: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  5. Marcilio F Lemos: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  6. Antonio C Costa: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil. ORCID
  7. Adriana P Compri: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  8. Yasmin França: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  9. Ellen Viana: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  10. Fernanda Malta: Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil.
  11. Roberta S Medeiros: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  12. Raquel Guiducci: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  13. Simone G Morillo: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
  14. Michele S Gomes-Gouvea: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil.
  15. Deyvid Amgarten: Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil.
  16. João R R Pinho: Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil. ORCID
  17. Regina C Moreira: Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil. ORCID
PMID: 36851548 DOI: 10.3390/v15020335

Abstract

Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in São Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7-93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide.

Keywords

Hepatitis E virus genotype 3 phylogeny public health zoonosis

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MeSH Term

Humans
Animals
Swine
Brazil
Hepatitis E virus
Rodentia
Feces
Genotype
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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