Inactivation of Human Norovirus GII.4 and in the Sea Squirt () by Floating Electrode-Dielectric Barrier Discharge Plasma.
Min Gyu Song, So Hee Kim, Eun Bi Jeon, Kwang Soo Ha, Sung Rae Cho, Yeoun Joong Jung, Eun Ha Choi, Jun Sup Lim, Jinsung Choi, Shin Young Park
Author Information
Min Gyu Song: Department of Seafood Science and Technology, Gyeongsang National University, Tongyeong 53064, Republic of Korea.
So Hee Kim: Department of Seafood Science and Technology, Gyeongsang National University, Tongyeong 53064, Republic of Korea.
Eun Bi Jeon: Department of Seafood Science and Technology, Gyeongsang National University, Tongyeong 53064, Republic of Korea.
Kwang Soo Ha: Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science, Tongyeong 53085, Republic of Korea.
Sung Rae Cho: Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science, Tongyeong 53085, Republic of Korea.
Yeoun Joong Jung: Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
Eun Ha Choi: Department of Electrical and Biological Physics, Plasma Bioscience Research Center, Kwangwoon University, Seoul 01987, Republic of Korea. ORCID
Jun Sup Lim: Department of Electrical and Biological Physics, Plasma Bioscience Research Center, Kwangwoon University, Seoul 01987, Republic of Korea. ORCID
Jinsung Choi: Department of Electrical and Biological Physics, Plasma Bioscience Research Center, Kwangwoon University, Seoul 01987, Republic of Korea. ORCID
Shin Young Park: Department of Seafood Science and Technology, Gyeongsang National University, Tongyeong 53064, Republic of Korea. ORCID
Human norovirus (HNoV) GII.4 and may be found in sea squirts. Antimicrobial effects of floating electrode-dielectric barrier discharge (FE-DBD) plasma (5-75 min, N 1.5 m/s, 1.1 kV, 43 kHz) treatment were examined. HNoV GII.4 decreased by 0.11-1.29 log copy/μL with increasing duration of treatment time, and further by 0.34 log copy/μL when propidium monoazide (PMA) treatment was added to distinguish infectious viruses. The decimal reduction time (D) of non-PMA and PMA-treated HNoV GII.4 by first-order kinetics were 61.7 (R = 0.97) and 58.8 (R = 0.92) min, respectively. decreased by 0.16-1.5 log CFU/g as treatment duration increased. The D for by first-order kinetics was 65.36 (R = 0.90) min. Volatile basic nitrogen showed no significant difference from the control until 15 min of FE-DBD plasma treatment, increasing after 30 min. The pH did not differ significantly from the control by 45-60 min, and Hunter color in "L" (lightness), "a" (redness), and "b" (yellowness) values reduced significantly as treatment duration increased. Textures appeared to be individual differences but were not changed by treatment. Therefore, this study suggests that FE-DBD plasma has the potential to serve as a new antimicrobial to foster safer consumption of raw sea squirts.
