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Radiation research
04 01, 2023;199 (4): 406-421.

Participation of ATM, SMG1, and DDX5 in a DNA Damage-Induced Alternative Splicing Pathway.

Jennifer J McCann, Donald E Fleenor, Jing Chen, Chun-Hsiang Lai, Thomas E Bass, Michael B Kastan
Author Information
  1. Jennifer J McCann: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
  2. Donald E Fleenor: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
  3. Jing Chen: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
  4. Chun-Hsiang Lai: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
  5. Thomas E Bass: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
  6. Michael B Kastan: Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina 27710.
PMID: 36921295 DOI: 10.1667/RADE-22-00219.1

Abstract

Altered cellular responses to DNA damage can contribute to cancer development, progression, and therapeutic resistance. Mutations in key DNA damage response factors occur across many cancer types, and the DNA damage-responsive gene, TP53, is frequently mutated in a high percentage of cancers. We recently reported that an alternative splicing pathway induced by DNA damage regulates alternative splicing of TP53 RNA and further modulates cellular stress responses. Through damage-induced inhibition of the SMG1 kinase, TP53 pre-mRNA is alternatively spliced to generate TP53b mRNA and p53b protein is required for optimal induction of cellular senescence after ionizing radiation-induced DNA damage. Herein, we confirmed and extended these observations by demonstrating that the ATM protein kinase is required for repression of SMG1 kinase activity after ionizing radiation. We found that the RNA helicase and splicing factor, DDX5, interacts with SMG1, is required for alternative splicing of TP53 pre-mRNA to TP53b and TP53c mRNAs after DNA damage, and contributes to radiation-induced cellular senescence. Interestingly, the role of SMG1 in alternative splicing of p53 appears to be distinguishable from its role in regulating nonsense-mediated RNA decay. Thus, ATM, SMG1, and DDX5 participate in a DNA damage-induced alternative splicing pathway that regulates TP53 splicing and modulates radiation-induced cellular senescence.

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Grants

  1. K00 CA212225/NCI NIH HHS
  2. P30 CA014236/NCI NIH HHS
  3. R01 ES005777/NIEHS NIH HHS

MeSH Term

Humans
Alternative Splicing
Protein Serine-Threonine Kinases
RNA Precursors
DNA Damage
Neoplasms
DEAD-box RNA Helicases
Ataxia Telangiectasia Mutated Proteins

Chemicals

Protein Serine-Threonine Kinases
RNA Precursors
Ddx5 protein, human
DEAD-box RNA Helicases
SMG1 protein, human
ATM protein, human
Ataxia Telangiectasia Mutated Proteins
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
Article has an altmetric score of 4

Word Cloud

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